Hampp (J. Am. Dental Assoc. 34:317, 1947) reported the successful recovery of six of seven spiral organisms from lyophilized material with the use of a complex medium unavailable through commercial sources. Annear (J. Bacteriol. 83:932, 1962; J. Gen. Microbiol. 27:341, 1962) recovered Reiter Treponeme and four strains of Leptospira from dried material by use of a prolonged and complex method. E...

The objective of this experiment is to formulate the tigecycline injection by lyophillization technique for better stability and for long term storage. Lactose monohydrate and lactose sprat dried were used with water for injection and hydrochloric acid into 5 ml tubular vials with pre and post purging nitrogen. The filled vials were loaded into lyophilizer and lyophilized them as per cycle. Dif...

Cyclohexanone monooxygenase (CHMO) was used as a model enzyme to find suitable freeze-drying conditions for long-term storage of an isolated monooxygenase. CHMO is a Baeyer-Villiger monooxygenase (BVMO) known for its ability to catalyze a large number of oxidation reactions. With a focus on establishing the optimal formulation, additives were tested for enzyme stabilization during and after lyo...

Lyophilization (freeze-drying) is one of the most suitable methods used for a long term peservation of pathogens. The aim of this paper was the application of lyophilization for storage of three significant plant pathogens: Fusarium graminearum, Helminthosporium gramineum, and Pseudomonas syringae pv. gylicinea, respectively. The plant material was collected continuously (during a four year per...

A cold plasma ionization device has been designed to monitor freeze-drying processes in situ by monitoring lyophilization chamber moisture content. This plasma device, which consists of a probe that can be mounted directly on the lyophilization chamber, depends upon the ionization of nitrogen and water molecules using a radiofrequency generator and spectrometric signal collection. The study per...

Human Milk (HM) is the optimal source of nutrition for preterm newborns (PTNBs). Still, adapting appropriate nutrient supply these babies a major challenge. Lyophilization common procedure employed to conserve HM that enables reconstitution milk in different volumes, therefore it provides better control content, and consequently facilitates its nutritional content fulfill needs PTNBs. Thus, thi...

PURPOSE Nanosuspension in drug delivery is known to improve solubility, dissolution and eventually bioavailability of the drugs. The purpose of the study was to compare particle size of nanosuspensions prepared by the first generation approach and H96 approach and to evaluate the effectiveness of H96 approach. METHODS The nanosuspension of aprepitant was prepared by HPH and H96 approach. The ...

Knowledge on long-term preservation of micro-organisms is limited and research in the field is scarce despite its importance for microbial biodiversity and biotechnological innovation. Preservation of fastidious organisms such as methane-oxidizing bacteria (MOB) has proven difficult. Most MOB do not survive lyophilization and only some can be cryopreserved successfully for short periods. A larg...