aril browning threatens production, consumption, and exports of pomegranates, because affected fruit cannot be externally distinguished from healthy fruit. this study compared the mineral, biochemical composition, and related enzyme activities in affected brown arils with healthy ones in ‘malase saveh’ pomegranates. the results indicated that concentrations of cu in the aril and k, mg, and mn i...

Tomato Lycopersicon esculentum Mill. is one of the most cultivated and widely consumed vegetables in world. However, it very susceptible to infection initiated by Fusariumoxysporum fruit rot, which shortens post-harvest life thus reduces market value. This disease can be regulated appropriately application synthetic fungicides. chemical fungicides constitute a serious health risk, have harmful ...

L-Phenylalanine is one of the essential amino acids that cannot be synthesized in mammals in adequate amounts to meet the requirements for protein synthesis. Fungi and plants are able to synthesize phenylalanine via the shikimic acid pathway. L-Phenylalanine, derived from the shikimic acid pathway, is used directly for protein synthesis in plants or metabolized through the phenylpropanoid pathw...

Glyphosate showed a remarkable effect inducing the change of flower symmetry from the actinomorphic to the zygomorphic type in Petunia hybrida. Glyphosate [N-(phosphonomethyl)glycine] reduced the anthocyanin content and showed a weak inhibitory effect against phenylalanine ammonia-lyase (PAL) activity. L-2-Aminooxy-3-phenylpropionic acid (APA), an inhibitor of PAL activity, reduced the anthocya...

Metabolic channeling has been proposed to occur at the entry point into plant phenylpropanoid biosynthesis. To determine whether isoforms of L-Phe ammonia-lyase (PAL), the first enzyme in the pathway, can associate with the next enzyme, the endomembrane-bound cinnamate 4-hydroxylase (C4H), to facilitate channeling, we generated transgenic tobacco (Nicotiana tabacum) plants independently express...

The influence of maturing and ripening on phenylalanine ammonia-lyase (PAL) activity and anthocyanin accumulation was examined using bagged, non-bagged and shaded ‘Jonathan’ (Malus domestica) apples. Bagged fruit were covered with paper bags from mid-June at an early stage of fruit growth. Shaded apples were insulated from sunlight by newspaper at the beginning of colouring (middle September). ...

To investigate the lignifications process and its physiological significance under Karnal Bunt (KB), the changes in enzymes responsible for lignifications likes, phenylalanine ammonia lyase (PAL), were determined in resistant (HD-29) and susceptible genotype (WH-542) of wheat during different developmental stages. The PAL gene was cloned and sequenced. The expression of PAL gene was measured by...

Phenylalanine ammonia-lyase (PAL) performs a major role in the biosynthesis of polyphenolic compounds, which are involved in the defense mechanism in harsh environments related to different stimuli. The present study was carried out to detect the activity of phenylalanine ammonia-lyase in different plant parts of Anacardium occidentale such as flower, young leaves, shoot tip, mature leaves, raw...

In a series of experiment, the response of two contrasting potato species, namely,  Solanum acaule (tolerant) and Solanum tuberosum cv. Agria (intolerant) to salt (80 mM NaCl) and drought stress (15% polyethylene glycol;PEG) was studied in vitro. Furthermore, the role of acetyl salicylic acid (ASA) (1 and 10 µM) in alleviating oxidative stress was investigated. In Agria cultivar, NaCl and PEG r...

Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Kelch domain-containing F-box proteins, AtKFB01, AtKFB20, and AtKFB50, function ...