Background/Introduction Lung isolation, a technique largely used to facilitate access during thoracic surgery, can create some difficulty in maintaining a patient’s blood gas balance. Strategies have been used to overcome the ventilation-perfusion mismatch associated with one-lung ventilation (OLV). However, such strategies may induce volutrauma, barotrauma and atelectotrauma in the ventilated ...

We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung cell suspensions through magnetic-based negative selection using a Biotin-antibody, Streptavidin-...

The isolation and identification of the 87A strain of epidemic hepatitis E virus (HEV) by means of cell culturing have been described previously. This paper reports the successful isolation of a sporadic HEV strain (G93-2) in human lung carcinoma cell (A549) cultures. The etiology, molecular and biological properties, and serological relationship of this new strain to other, epidemic HEV strain...

New discoveries in stem cell biology are making the biology of solid tissues increasingly complex. Important seminal studies demonstrating the presence of damage-resistant cell populations together with new isolation and characterization techniques suggest that stem cells exist in the adult lung. More detailed in vivo molecular and cellular characterization of bronchioalveolar stem cells (BASCs...

BACKGROUND Co-existence or subsequent isolation of multiple nontuberculous mycobacteria (NTM) species in same patient has been reported. However, clinical significance of these observations is unclear. The aim of this study was to determine clinical implications of changes of NTM species during or after treatment in patients with NTM lung disease. METHODS Patients with NTM lung disease, who e...

PURPOSE To describe the anesthetic management of a patient with previous left lower lobe resection who was submitted to a right upper lobectomy and review the changes in gas exchange and respiratory mechanics which occurred intraoperatively. CLINICAL FEATURES A 69-yr-old male with lung cancer, emphysema and obstructive sleep apnea, presented for a right upper lobectomy. His history was also p...

introduction: the respiratory system is both a route of entry and exit for toxins and injurious agents, as well as being a target for chemical substances and pathogens. therefore, an understanding of the structure and function of the migratory cell populations of pulmonary tissues including alveolar macrophages is central in a number of important disease processes. this study aimed to identify ...

lung exposure to sulfur mustard (sm) results in pulmonary complications, which is the main cause of long-term disability and morbidity. up to now, the precise mechanisms of sm-induced lung complications has not been identified. the aim of this study was to evaluate apoptosis in the lung tissue of sm-exposed individuals. the study was performed on archived lung paraffin-embedded tissue specimens...

The intrinsic anatomical complexity of the lung, its slow cell turnover, and the lack of regenerative models are among the factors that have complicated the study and isolation of adult lung stem cells. Despite this, several endogenous lung progenitor cells have been identified in the proximal and distal lung. However, there is limited data regarding the lineage relationships, self-renewal prop...

A new and as yet unidentified bacterium was isolated from the lung tissue of a cancer patient with bilateral pneumonia. Clinically, the pneumonia was consistent with legionellosis; the organism cultured from the lung grew only on the charcoal-yeast extract agar routinely used for Legionella isolation. Subsequent testing, however, showed the organism to be quite distinct from the known Legionell...