Metabarcoding has become an important tool in the discovery of biodiversity, including fungi, which are the second most speciose group of eukaryotes, with diverse and important ecological roles in terrestrial ecosystems. We have designed and tested new PCR primers that target the D1 variable region of nuclear large subunit (LSU) ribosomal DNA; one set that targets the phylum Ascomycota and anot...

ii ACKNOWLEDGEMENTS I would like to dedicate my work to my parents, Mr. and Mrs. Chamkura Padma Rao, my brother Aravind and my sister Jyothi, for their constant prayers and encouragement throughout my life. I am very grateful to my advisor Dr. A. Srivastava for his guidance, patience and understanding throughout this work. His suggestions, discussions and constant encouragement have helped me t...

Vegetation crop marks may be formed in areas where vegetation overlays near-surface archaeological remains. These features retain soil moisture with different percentage of moisture compared to the rest of the crops of an area. Depending on the type of feature, crop vigour may be enhanced or reduced by buried archaeological features. Satellite imagery has been already applied successfully in se...

Mammalian mitochondria harbor a dedicated translation apparatus that is required for the synthesis of 13 mitochondrial DNA (mtDNA)-encoded polypeptides, all of which are essential components of the oxidative phosphorylation (OXPHOS) complexes. Little is known about the mechanism of assembly of the mitoribosomes that catalyze this process. Here we show that C7orf30, a member of the large family ...

The complete or nearly complete large-subunit rRNA (LSU rRNA) sequences were determined for representatives of several algal groups such as the chlorarachniophytes, cryptomonads, haptophytes, bacillariophytes, dictyochophytes and pelagophytes. Our aim was to study the phylogenetic position and relationships of the different groups of algae, and in particular to study the relationships among the...

Amomum villosum Lour., produced from Yangchun, Guangdong Province, China, is a Daodi medicinal material of Amomi Fructus in traditional Chinese medicine. This herb germplasm should be accurately identified and collected to ensure its quality and safety in medication. In the present study, single nucleotide polymorphism typing method was evaluated on the basis of DNA barcoding markers to identif...

OBJECTIVE To examine the validity and clinical utility of functional assessments (1-minute walk test, 10-meter walk test, Timed Up & Go [TUG] test, Timed Up and Down Stairs [TUDS] test, sit-to-stand [STS] test, and lateral step-up [LSU] test). DESIGN Cross-sectional study. SETTING Four special schools for adolescents with physical disabilities. PARTICIPANTS Adolescents with spastic tetrap...