This study aimed to study the role of rapamycin (RAPA) in modulating the interaction between gδ T cells and dendritic cells (DCs) in a lipopolysaccharide (LPS)-induced acute lung injury mouse model. Mice were injected with LPS to establish the acute lung injury model or LPS + RAPA to assess the role of RAPA in modulating cell interactions. Mice were injected with PBS or RAPA alone as controls. ...

Objective(s): TLR-4 activates a number of inflammatory signaling pathways. Also, AMPK could be involved in anti-inflammatory signaling. The aim of this study was to identify whether stimulation of AMPK could inhibit LPS-induced Tlr-4 gene expression in mice hearts. Materials and methods: Heart AMPK activity and/or Tlr-4 expression was stimulated in different mice groups, using respectively IP i...

Objective: To investigate how dihydroartemisinin (DHA) modulate Toll-like receptors-4 signaling pathway in LPS-stimulated RAW264.7 murine. Methods: IRF3 was detected in RAW264.7 cells treated with lipopolysaccharide (LPS) (1 μg/ml), lipopolysaccharide and dihydroartemisinin (LPS/DHA) (10 μM) and equal volume of culture medium respectively by using immunocytochemistry staining. IFN-α/β mRNA was ...

Objectives Platelet-rich plasma (PRP) plays an important role at all stages of wound healing, including the inflammatory stage. Macrophage autophagy has been found to influence response process. However, it is unclear whether PRP can affect responses via macrophage autophagy. In present study, we explored effect on and researched underlying mechanism. Methods RAW 264.7 macrophages were treated ...

Introduction: Gap junctions are intercellular membrane channels that provide direct cytoplasmic continuity between adjacent cells. This communication can be affected by changes in expression of gap junctional subunits called Connexins (Cx). Changes in the expression and function of connexins are associated with number of brain neurodegenerative diseases. Neuroinflammation is a hallmark of vario...

Background:
 Multiple Sclerosis (MS) is an autoimmune disease of the central nervous system (CNS). Pathogenic T cells, such as Th1 and Th17, infiltrate CNS, resulting in neuroinflammation, demyelination axonal damage. activates microglia (MG) CNS Th17 acts a chemokine to recruit immune cells into CNS. MG resident cell its activation associated with destruction myelin secretion inflammatory...

BACKGROUND/AIM Several reports have indicated that environmental factors and defects in innate immunity are central to the pathogenesis of inflammatory bowel disease (IBD). Although bacteria producing lipopolysaccharide (LPS), which is a Toll-like receptor (TLR) 4 agonist, play a crucial role in the development of experimental colitis, LPS tolerance following initial exposure to LPS can result ...

Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. In this study, we investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA expression...

The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) often causes secondary bacterial infection in piglets, resulting inflammatory lung injury leading to high mortality rates significant economic losses the pig industry. Microvascular endothelial cells (MVECs) play a crucial role response. Previous studies have shown that HP-PRRSV can infect pulmonary MVECs damag...

Fibroblast-like synoviocytes (FLSs) produce lots of inflammatory molecules that trigger immune responses and intensification the inflammation thereby play important roles in Rheumatoid Arthritis )RA( pathogenesis. Due to toll-like receptor 4 (TLR4) cytokine production inflammation, we aimed evaluate effects TAK-242 (Resatorvid) on interleukin (IL)1-β, IL-6, TNF-α, TLR4 expression two proteins n...