We report the structure activity relationships of short 14-mer phosphorothioate gapmer antisense oligonucleotides (ASOs) modified with α-L-locked nucleic acid (LNA) and related modifications targeting phosphatase and tensin homologue (PTEN) messenger RNA in mice. α-L-LNA represents the α-anomer of enantio-LNA and modified oligonucleotides show LNA like binding affinity for complementary RNA. In...

This paper presents a 5.8 GHz low voltage and low power LNA design integrated in a TSMC 0.18 μm CMOS process, and its sensitivity analysis. This sensitivity analysis gives a measure of the sensitivity of the LNA performance to a change in the circuit element values, thereby assisting the designer choose the adequate circuit-element tolerances. Such sensitivity analysis of the LNA is very benefi...

RNA antagonists using locked nucleic acid (LNA) antisense oligonucleotides are among the most promising therapeutics to treat cancer. Unlike the other antisense oligonucleotides, LNA-based oligonucleotides have high target binding affinity and long tissue stability. LNA can inhibit the mRNA expression at nanomolar and subnanomolar concentrations in the presence of lipofectamine. However, system...

This paper presents an inductorless low-noise amplifier (LNA) design for an ultra-wideband (UWB) receiver frontend. A current-reuse gain-enhanced noise canceling architecture is proposed, and the properties and limitations of the gainenhancement stage are discussed. Capacitive peaking is employed to improve the gain flatness and 3-dB bandwidth, at the cost of absolute gain value. The LNA circui...

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) may be biosynthesized from a precursor alpha-linolenic acid (LNA) or obtained preformed in the diet. Dams were fed four diets with different levels of the various n-3 fatty acids during pregnancy and lactation, and their offspring were weaned to the same diets: "n-3 Deficient," containing (as % total fatty acids) 0.07% of LNA; "Low LNA"...

We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to...

Locked nucleic acid (LNA) oligonucleotides have been successfully used to efficiently inhibit endogenous small noncoding RNAs in vitro and in vivo. We previously demonstrated that the direct miR-221 inhibition by the novel 13-mer LNA-i-miR-221 induces significant antimyeloma activity and upregulates canonical miR-221 targets in vitro and in vivo. To evaluate the LNA-i-miR-221 pharmacokinetics a...

Substrate noise generated by digital circuits on mixed-signal ICs can disturb the sensitive analog/RF circuits, such as Low Noise Amplifier (LNA), sharing the same substrate. This paper investigates the adverse impact of the substrate noise on a high frequency cascode LNA laid out on a lightly doped substrate. By studying the major noise coupling mechanisms, a new and efficient modeling method ...

We have determined the NMR structure of an intramolecular dsDNA:LNA triplex, where the LNA strand is composed of alternating LNA and DNA nucleotides. The LNA oligonucleotide binds to the dsDNA duplex in the major groove by formation of Hoogsteen hydrogen bonds to the purine strand of the duplex. The structure of the dsDNA duplex is changed to accommodate the LNA strand, and it adopts a geometry...

Aptamers constitute a potential class of therapeutic molecules typically selected from a large pool of oligonucleotides against a specific target. With a scope of developing unique shorter aptamers with very high biostability and affinity, locked nucleic acid (LNA) nucleotides have been investigated as a substrate for various polymerases. Various reports showed that some thermophilic B-family D...