Isotope dilution mass spectrometry with liquid chromatography for the determination of serum creatinine is studied. After adding labeled (15N2) creatinine to the serum, creatinine was isolated using high performance liquid chromatography. Measurements were performed by electron impact mass spectrometry with use of a direct insertion device. Analytical results of the NIST (formerly NBS) SRM seru...

Citation: Zhang X, Li J, Xie B, Wu B, Lei S, Yao Y, He M, Ouyang H, Feng Y, Xu W and Yang S (2018) Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry. Front. Pharmacol. 9:282. doi: 10.3389/fphar.2018.00282 Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Muci...

Mass spectrometry has long been used in clinical laboratories. The first important applications were the use of gas chromatography–mass spectrometry (GC-MS) for drug screening and the diagnosis of organic acid metabolic disorders. More recently liquid chromatography–mass spectrometry [LC-MS; liquid chromatography–tandem mass spectrometry (LC-MS/MS)] has been introduced successfully into clinica...

Chromatography coupling to mass spectrometry becomes a vital instrumentation tool for the separation and determination of small molecules qualitatively and quantitatively. In this study, the author is focused on the mitragynine and 7hydroxymitragynine. Mitragynine and its major metabolite, 7-hydroxymitragynine (also known as “kratom”), are drugs which extracted from a tree planted in Thailand, ...

A retention prediction model was developed for peptides separated in reversed-phase chromatography. The model was utilized to identify and exclude the false positive (FP) peptide identifications obtained via database search. The selected database included human proteins, as well as decoy sequences of random proteins. The FP peptide detection rate was defined either as number of retention time o...

Today, 2D online or offline liquid chromatography/mass spectrometry is state of the art for the identification of proteins from complex proteome samples in many laboratories. Both 2D liquid chromatography methods use two orthogonal liquid chromatography separation techniques. The most commonly used techniques are strong cation exchange chromatography for the first dimension and reversed phase s...

The potential of ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI-MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric ...