This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully a...

background: diarrhea, caused by enteroaggregative escherichia coli (eaec), is an important cause of illness and death. numerous virulent factors have been described in eaec. however, their prevalence was highly variable among eaecs of distinct geographic locations. studies have shown that dispersin (antiaggregation protein, aap) is one of the important and abundant virulent factors in eaec. in ...

introduction: periodontitis is a multi-factorial inflammatory disease of periodontium. a precise mechanism in which smoking affects periodontium is not accurately understood. this study was planned to survey salivary osteocalcin (oc) level among smokers with chronic periodontitis and its comparison to non-smoker patients which may help achieve a better understanding of mechanisms being involved...

ELISA: enzyme-linked immunosorbent assay IIF: indirect Immunofluorescence PNP: paraneoplastic pemphigus PV: pemphigus vulgaris INTRODUCTION Because of variability in clinical and histopathologic findings, the diagnosis of paraneoplastic pemphigus (PNP) frequently relies on the combination of direct immunofluorescence and indirect immunofluorescence (IIF). Here we present a unique case of PNP as...

The enzyme, xanthine oxidase, has been implicated as a major source of destructive free radicals in ischemia-reperfusion injury associated with a range of pathological states [l]. Enzymic activity has been detected in many mammalian tissues [4] by using, variously, spectrophotometric [51, radiometric[6] and fluorimetric [71 assays. Apart from bovine milk fat globule membrane, which has long pro...