BACKGROUND Lactobacillus plantarum is an attractive candidate for metabolic engineering towards bioprocessing of lignocellulosic biomass to ethanol or polylactic acid, as its natural characteristics include high ethanol and acid tolerance and the ability to metabolize the two major polysaccharide constituents of lignocellulolytic biomass (pentoses and hexoses). We recently engineered L. plantar...

The supplementation of lignocellulose-degrading enzymes can be used to enhance the performance biogas production in industrial plants. Since structural stability these enzyme preparations is essential for efficient application, reliable methods assessment are crucial. Here, a mass-spectrometric-based assay was established monitor enzymes, i.e., integrity proteins, anaerobic digestion (AD). anal...

Carbon, hydrogen and nitrogen analyses of banana leaf and pseudostem biomass revealed their potentiality as substrates for microorganisms. Infra-red (IR) spectra of both biomass show presence of cellulose, xylan and lignin. IR spectra of leaf and pseudostem biomass degraded in solid state fermentation (SSF) by two Pleurotus species (P. sajor-caju and P. ostreatus) for 40 days showed the utiliza...

Degradation of lignocellulosic materials to release fermentable mono- and disaccharides is a decisive step toward sustainable bio-based economy, thereby increasing the demand robust highly active lignocellulolytic enzymes. Anaerobic fungi phylum Neocallimastigomycota are potent biomass degraders harboring huge variety such Compared cellulose, hemicellulose degradation has received much less att...

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated Klebsiella sp. strain BRL6-2 on minimal media with alkali lignin as the sole carbon source. This organism was isolated anaerobically from tropical forest soils collected from the Bisley watershed at the Ridge site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological ...

Nowadays, the discovery of lignocellulolytic microorganisms that are better adapted to operational conditions while exhibiting the strong degrading activities is highly desired for successful lignocellulose biotransformation processes. In this study, microorganisms were isolated from lignocellulose-rich composting materials by selective methods. A screening of isolates known to have lignocellul...

Recent studies have described the bacterial community residing in the guts of giant pandas, together with the presence of lignocellulolytic enzymes. However, a more comprehensive understanding of the intestinal microbial composition and its functional capacity in giant pandas remains a major goal. Here, we conducted a comparison of bacterial, fungal and homoacetogenic microbial communities from...