PURPOSE The purposes of this study were to investigate the immunostaining of the leukocyte adhesion molecules intercellular adhesion molecule (ICAM)-1 and E-selectin in subfoveal choroidal neovascular membranes (CNVMs) surgically excised from patients with age-related macular degeneration (AMD) and to determine whether prior photodynamic therapy (PDT) alters their immunostaining. METHODS The ...

Inflammatory bowel disease (IBD) is a chronic disorder whose etiology is linked to triggering events, including viral infections, that lead to immunoregulatory dysfunction in genetically susceptible people. Characteristic pathological changes include increased mononuclear leukocyte influx into the intestinal mucosa as well as mucosal smooth muscle cell (M-SMC) hyperplasia. Virus infection or vi...

This study was performed to define the correlation between the level of consciousness based on Glasgow coma scale ( GCS), Glasgow outcome scale( GOS) and leukocyte count. Two hundred and thirty three patients ( female: 48, male: 185) ranged between 1-75 years old were studied. The results showed that there was a negative correlation between increasing leukocyte count and  GCS ( n=233.r= .0.3, p...

background: miscarriage is a common phenomenon complicating more than half of pregnancies. recurrent pregnancy loss (rpl) is defined as three or more pregnancies lost before the twentieth week of gestation. it is believed that abnormality in maternal immune reaction to fetus and sharing of hla antigens might be associated with rpl. objective: to investigate the effect of hla-drb1 sharing betwee...

Psoriasis is a chronic inflammatory skin disorder characterized by hyperproliferation and aberrant differentiation of keratinocytes, inflammation in dermis epidermis leukocyte infiltration Th1-like cytokine pattern. An experimental mechanism-based vitro model has been developed using novel multi-compartment reconstructed full-thickness (3C System Episkin SA) applying systemically mixture Th-1 c...

Fish have habitats that are easily exposed to contamination from the environment such as heavy metal waste which can cause changes in structure and function of body tissues. One type enters river Lebong Regency River is gold mining processing uses mercury cyanide. Heavy metals enter organisms accumulation biomagnification occur bodies aquatic biota fish. There has been no research related fish ...

A panel of 377 commercially available mAbs were submitted to the animal homologue section of the 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) for cross-reactivity studies on different animal species. In this study we describe the results of testing the mAbs on cattle cells by flow cytometry and Western blot. Eight commercial suppliers parti...

We describe a microfluidic device for on-chip chemical processing, such as staining, and subsequent washing of cells. The paper introduces "separator walls" to increase the on-chip incubation time and to improve the quality of washing. Cells of interest are concentrated into a treatment stream of chemical reagents at the first separator wall for extended on-chip incubation without causing exces...

Methods. Cryostat sections of human retina were overlaid with blood lymphocytes from healthy subjects, and experimental conditions were sought by which preferential attachment of the cells occurred to blood vessel walls in the retinal sections. Adherent lymphocytes were identified by staining with methyl green-thionine, and transected blood vessels were identified by their structure and by stai...

Studies by Wagner (1) on the quantitative estimation of glycogen in human blood disclosed the average glycogen content per 100 ml. to be 5.5 mg. In separated leukocytes an average of 2.54 micrograms of glycogen was found per 106 total leukocytes, or 4.23 micrograms per 106 granulocytic leukocytes. No detectable glycogen is present in human plasma or erythrocytes. It has been both denied (2) and...