نتایج جستجو برای: lentiviral vector

تعداد نتایج: 200877  

2014
Ulrike Mock Kristoffer Riecken Belinda Berdien Waseem Qasim Emma Chan Toni Cathomen Boris Fehse

TAL-effector nucleases (TALENs) are attractive tools for sequence-specific genome modifications, but their delivery still remains problematic. It is well known that the presence of multiple sequence repeats in TALEN genes hampers the use of lentiviral vectors. We report that lentiviral vectors readily package full-length vector mRNAs encoding TALENs, but recombination during reverse transcripti...

Journal: :Molecular Therapy - Methods & Clinical Development 2019

Journal: :Molecular therapy : the journal of the American Society of Gene Therapy 2017
Conrad A Vink John R Counsell Dany P Perocheau Rajvinder Karda Suzanne M K Buckley Martijn H Brugman Melanie Galla Axel Schambach Tristan R McKay Simon N Waddington Steven J Howe

Lentiviral vector genomic RNA requires sequences that partially overlap wild-type HIV-1 gag and env genes for packaging into vector particles. These HIV-1 packaging sequences constitute 19.6% of the wild-type HIV-1 genome and contain functional cis elements that potentially compromise clinical safety. Here, we describe the development of a novel lentiviral vector (LTR1) with a unique genomic st...

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