نتایج جستجو برای: ldh assay
تعداد نتایج: 229511 فیلتر نتایج به سال:
Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway
The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cyt...
In comparison to monolayer cells, MCTS has been claimed as more suitable candidate for studying drug penetration due to the high resemblance to solid tumors. However, the cultivation of MCTS is cumbersome, time consuming, and most technique fail to generate spheroids with uniform sizes. Therefore, the application of spheroid cultures in high throughput screening has been rather limiting. Beside...
The authors would like to draw attention an error in Figure 2 A of this Research Article. illustrations SnSe and LDH were incorrectly labeled. corrected version is provided Corrigendum. Also, the sentence “Figure shows that nanosheets preserve 84.6 % 64.7 catalytic activity their second fourth cycle uses, respectively. In contrast, loses 86.2 displays >99 inactivation at third cycle.” should re...
To explore the impact of the new derivatives cyclovirobuxine D (CBV-D) model of alcohol-induced injury in PC12 cells. The model of alcohol-induced injury in PC12 cells, new derivatives of the cyclovirobuxine D PC12 cells by MTT assay, Hoechst 33258 staining and lactate dehydrogenase (LDH) activity was measured. The new D derivatives cyclovirobuxine inhibit the damage caused by alcohol PC12 cell...
The aim of this study is to investigate the effects of taurine treatment on cell viability and proliferations in BHK-21 cells. In this study, BHK-21 cells were seeded at a density of 50.000/ml per well in 96 well plates in medium composed of Glasgow-Minimum Essential Medium (GMEM). The cells were either left untreated (control) or treated with different concentrations of taurine for 24 h. XTT c...
objective: nanosilver has numerous applications in medicine due to its potent antibacterial activity. however, data regarding the bio-safety of its effective concentrations is scarce. this study aims to assess the toxicity of silver nanoparticles on human gingival epithelial cells under in-vitro conditions. methods: this in vitro study evaluated the toxic effects of filtered and unfiltered na...
Nowadays, growing interest in the application of nanotechnology in biomedical and biotechnological fields has opened a new avenue to explore the nanoparticles-biological system interaction. Indeed, a clear gap is still in the cytotoxic effect of NPs on the biological systems. For this purpose, the interaction of the silica oxide nanoparticles (SiO2-NP) with PC12 cell line, as a model of nervous...
METHODS. Cellular reductive capacity was evaluated using WST-1 tetrazolium salt. Mitochondrial transmembrane potential was determined by JC-1 fluorescence. Cellular ATP levels were measured with a luciferase assay. Caspase-3/7 activation was detected with a DEVDase activity assay. Cell death and lysis was evaluated by measuring release of lactate dehydrogenase (LDH). Glycolysis was assessed by ...
Breast cancer is the leading cause of death in women worldwide. The aim this study was to determine value post-therapy serum LDH assessing response at completion chemotherapy breast patients. This a prospective cross-sectional with data collection from May 2019 November 2020, University Hospital Bogodogo and SANDOF Polyclinic. We performed assay on patients end who were least 18 years old. Thir...
The problem of real treatment teeth hypersensitivity is still important and unsolved. main goal the experiment was to calculate possible toxic effects on fibroblasts cells CCL-1™ (NCTC clone 929) caused by original preparation reduce tooth surfaces’ hypersensitivity, compared marketable Seal & Protect (Dentsply). assessment made through measuring lactate dehydrogenase (LDH assay). Lactate r...
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