Cytokine production in response to allergens may influence the development of atopy-predisposing immune responses, initializing the early programming of allergy and asthma. Vitamin D intake may be protective due to its immunoregulatory properties, that may contribute to influence the expression of the atopic phenotype initiated in early life. The objective of our study was to investigate the ef...

Milk elimination diets are frequently adopted in the treatment of atopic dermatitis, although the real prevalence of clinically relevant food allergy remains unclear and reports from different authors are often in disagreement. We investigated the percentage of children allergic to cow's milk compared with the rate of milk exclusion diets in a group of patients with atopic dermatitis. We enroll...

BACKGROUND We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector cell activation by nitrated food proteins. OBJECTIVE The aim of this study was to investigate the influence of pro...

Comparing lymphocyte responses to allergenic and nonallergenic foods could reveal the differences between pathogenic and normal immune responses to foods. Defining the cytokine-producing phenotypes of peanut-specific lymphocytes from peanut-allergic children, children who outgrew peanut allergy, and children who have always tolerated peanuts may be useful for understanding the mechanisms of foo...

Abstract The most allergenic protein in cow’s milk for new-borns is Beta Lactoglobulin (β-LG), which also one of the bovine whey proteins. As a result, choosing suitable animals to create free essential. In this study, Indonesian Friesian Holstein’s genetic variants β-LG gene were examined). Forty-two blood samples sampled as DNA sources. allele and genotype variant identified using PCR-RFLP, o...

The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. There is very little incorporation of amines in the absence of reducing agent. By isolating and sequencing the modified ...

The concentrations of beta-lactoglobulin was measured in the sera of 47 preterm an term neonates during the first few days of life under standardised conditions after feeding with a cows' milk'based formula. Preterm neonates, particularly those of less than 33 weeks' gestation, had higher serum concentrations of beta-lactoglobulin than term neonates given an equivalent mild feed. Prior feeding ...

as 'goat lactoglobulin' gave a single peak on electrophoresis at pH 8.0 in barbiturate buffer, but three boundaries separated at pH 7-0 and at least two at pH 4-2-6-0 after 2 hr. at 4-5 v/cm., I= 01. The two main components had their isoelectric points at pH 5-2 and 5-9 respectively in sodium acetate buffer, I= 0-1. 'Goat lactoglobulin' thus behaved in much the same way as crystalline bovine ,B...

Depending on solution conditions, beta-lactoglobulin can exist in one of its six pH-dependent structural states. We have characterized the acid and basic-induced conformational transitions between these structural states over the pH range of pH 1 to pH 13. To this end, we have employed high-precision ultrasonic and densimetric measurements coupled with fluorescence and CD spectroscopic data. Ou...