ThirtyFrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. Polymorphism of cow lactoferrin (LTF) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that LFT gene pro...

The transition period for dairy cows is generally defined as the time period from three weeks prior to parturition through three weeks after parturition. It is now recognized that defining and meeting the nutritional requirements of the transition dairy cow can greatly impact animal health, production in the ensuing lactation, overall longevity, and animal well-being (NRC, 2001). Nutrition and ...

For the rapid and accurate identification of cow reproduction and healthy behavior from mass surveillance video, in this study, 400 head of young cows and lactating cows were taken as the research object and analyzed cow behavior from the dairy activity area and milk hall ramp. The method of object recognition based on image entropy was proposed, aiming at the identification of motional cow obj...

The whole-animal model described in this paper is intended to be a research model with an intermediary structure between sophisticated and simple cow models. The mechanistic model structure integrates the main metabolic pathways of the lactating dairy cow. Milk yield and related feed intake for varying production potentials were considered to be the driving forces and were empirically defined. ...

Oil supplementation in dairy cattle diets is used to modulate milk fat composition, as well as the expression of mammary lipogenic genes, whose regulation remains unclear. MiRNAs are small non-coding RNA considered as crucial regulators of gene expression, offering clues to explain the mechanism underlying gene nutriregulation. The present study was designed to identify miRNAs whose expression ...

A study was conducted to assess the diversity among fecal Escherichia coli from healthy lactating cattle. E. coli (n = 100) isolates from 10 healthy lactating dairy cows of a Pennsylvania dairy herd were examined for phenotypic and genotypic characteristics. Results revealed 26, 58, 10, and 6 E. coli isolates belonged to phylogenetic groups A, B1, B2, and D respectively. Overall, 63 serotypes, ...

OBJECTIVE To estimate seroprevalence of Mycobacterium avium subsp paratuberculosis (MAP) infection among adult dairy cows in Colorado and determine herd-level factors associated with the risk that individual cows would be seropositive. DESIGN Cross-sectional observational study. ANIMALS 10,280 adult (> or = 2 years old) dairy cows in 15 herds in Colorado. PROCEDURE Serum samples were test...

Dairy cow health is multifactorial and complex. High producing dairy cows have been described as “metabolic athletes,” but 30 to 50% of cows are affected by a metabolic or infectious disease around calving (LeBlanc, 2010). Cows are highly susceptible to metabolic and infectious disease during the transition period, or the period from 3 weeks before to 3 weeks after calving (Huzzey et al., 2007,...