Six hundred lactating dairy buffaloes from four districts (Lahore, Sialkot, Narowal and Okara) of Punjab, Pakistan were screened for subclinical mastitis using White Side Test and subsequent bacterial isolation. Overall prevalence of subclinical mastitis was 44% (264/600). It was the highest (58%) in animals kept as individual holding at backyards followed by small holdings in periurban area (4...

This study evaluated the effect of a prostaglandin F2? (PGF) analogue as an ovulatory stimulus in dairy cows and buffaloes raised Amazon biome. To this end, three experiments were performed state Rondônia, located In Experiment 1, 22 lactating received 2 mg intramuscular (I.M.) estradiol benzoate (EB) on day 0 intravaginal progesterone-releasing device (CIDR) from to 9 protocol. On days 8 9, al...

The aim of the study was to produce sensitive and specifi c polyclonal antibodies against somatotropin (ST) to be used for standardization of an ELISA to measure growth hormone (GH) level in serum and milk of lactating buffaloes. Recombinant bovine somatotropin (rbST), kindly donated by NHPP, California, USA was injected both in chicken and rabbit emulsifi ed with Freund’s complete adjuvant fol...

The study was carried out to study individual test day variability in yield and composition of Surti and Jafarabadi buffaloes milk at day 15 and 60 postpartum (pp). 10 healthy Surti and Jafarabadi buffaloes of each breed were selected at random from Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, Gujarat, respectively. Buffaloes were further divided into four different g...

This study aimed to determine the haematological and biochemical changes that occur in buffaloes with parturient haemoglobinuria (PHU). For this purpose, serum samples from 60 PHU-affected and 60 apparently healthy buffaloes were collected and analysed. Mean erythrocyte count (3.6 ± 1.0 10/l), haemoglobin concentration (5.8 ± 1.4 g/dl), and haematocrit (16.9 ± 2.8 %) of the PHU-affected buffalo...

Effect of diameter of pre-ovulatory follicle (POF) and body condition score (BCS) of buffaloes on pregnancy rate following ovsynch-TAI were studied in 100 post-partum anestrus buffaloes. The size of POF was determined through sonography on day 10 of protocol. BCS of buffaloes were estimated at the time of AI. Pregnancy was con rmed per-rectally on day 60 post-AI. Pregnancy rate in buffaloes wi...