The genetic context of the blaNDM-1 gene in the genome of Pseudomonas aeruginosa MMA83 was investigated. Sequencing of the cosmid selected for the blaNDM-1 gene revealed the presence of two blaNDM-1 copies in the genome of P. aeruginosa MMA83 in a unique genetic environment. Additionally, mating assays, DNA-DNA hybridization, and an S1 nuclease assay strongly suggest that the blaNDM-1 gene in P...

The emergence and spread of NDM-1-producing Enterobacteriaceae have resulted in a worldwide public health risk that has affected some provinces of China. China is an exceptionally large country, and there is a crucial need to investigate the epidemic of blaNDM-1-positive Enterobacteriaceae in our province. A total of 186 carbapenem-resistant Enterobacteriaceae isolates (CRE) were collected in a...

genes. The PCR-based replicon typing method showed that this blaNDM-1-positive plasmid belonged to the IncHI incompatibility group. Genetic structures surrounding the blaNDM-1 gene were investigated using a PCR mapping approach and sequencing. The blaNDM-1 gene was immediately bracketed by a truncated insertion sequence ISAba125 upstream and the bleomycin resistance gene bleMBL downstream. This...

The acquisition of blaNDM-1 gene by Gram negative bacteria has emerged as critical threat to human health as these organisms display high resistance to available antibiotics. The presence of this gene has been observed in both clinical as well as environmental settings. The present study was carried out to evaluate the prevalence of blaNDM-1 containing microorganisms in water sources. A total o...

Carbapenems, 'last-resort' β-lactam antibiotics, are inactivated by zinc-dependent metallo-β-lactamases (MBLs). The host innate immune response withholds nutrient metal ions from microbial pathogens by releasing metal-chelating proteins such as calprotectin. We show that metal sequestration is detrimental for the accumulation of MBLs in the bacterial periplasm, because those enzymes are readily...

Members of the family Enterobacteriaceae include several human pathogens that can be acquired through contaminated food and water. In this study, the incidence of extended spectrum β-lactamase (ESBL)-producing enterobacteria was investigated in fresh seafood sold in retail markets. The ESBL-positive phenotype was detected in 169 (78.60%) isolates, with Escherichia coli being the predominant spe...

Sir, Since its discovery in 2008, the metallo-b-lactamase (MBL) NDM-1 has been identified in different Enterobacteriaceae species and recently also in a number of other bacterial species isolated from water supplies in India, such as Vibrio cholerae and Pseudomonas spp. Genes encoding NDM b-lactamases have been additionally identified in multidrugresistant Acinetobacter spp. Here, we report on ...

Here we describe carbapenem resistance determinants in two Klebsiella pneumoniae isolates recovered from two hospitalised patients in the same intensive care unit of a cancer hospital in Cairo, Egypt. PCR and sequencing were used to detect and characterise β-lactamase genes. Clonal relationships between the isolates were analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequenc...

OBJECTIVES To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, ...