Holstein bull calves (n = 51) weaned at 6 wk of age were used in four N balance trials for 4 wk (Trials 1 to 3) or for 2 wk (Trial 4) from 8 wk of age to identify limiting amino acids for a corn and soybean meal diet. The calves were trained to maintain reflex closure of the reticular groove throughout the trials. In Trial 1, administration of .111 g of DL-methionine plus .333 g of L-lysine mon...

Metabolism of L-[1-14C]methionine, DL-[1-14C]methionine and DL-[1-14C]2-hydroxy-4-methylthiobutanoic acid (DL-HMB) by broiler chicks which had been fasted overnight or given a methionine-deficient diet was compared with fed (control) birds. The excretion of 14C-labelled material, total 14CO2 exhaled, 14C incorporation into tissue proteins and the 14C-labelled material in perchloric-acid-soluble...

Tracing experiments were carried out to identify volatile and nonvolatile L-methionine degradation intermediates and end products in the yeast Geotrichum candidum and in the bacterium Brevibacterium linens, both of which are present in the surface flora of certain soft cheeses and contribute to the ripening reactions. Since the acid-sensitive bacterium B. linens is known to produce larger amoun...

Chronic administration of L-methionine leads to memory impairment, which is attributed to increase in the level of oxidative stress in the brain. On the other hand, metformin is a commonly used antidiabetic drug with strong antioxidant properties. In the current study, we tested if chronic metformin administration prevents memory impairment induced by administration of L-methionine. In addition...

L-methioninase degrades methionine, which is essential in methionine-dependent cancer cells, resulting specific cell death. Normal cells can synthesize their own methionine amino acids even the absence of exogenous methionine. This selective targeting makes a promising therapeutic candidate for cancer. In this study, was partially purified from Brevibacterium linens BL2. The activity enzyme fou...

An enzymatic assay for L-methionine was developed by coupling adenosylmethionine synthetase (AdoMetS) to a pyrophosphate (PP(i)) detection system, which was constructed using pyruvate, phosphate dikinase. To expand the use of this assay, the PP(i) detection system was embodied as three different forms, which allowed PP(i) to be measured by UV, visible, and fluorescent light detectors. The assay...