Split gill lamellae (epithelium plus cuticle) of hyperregulating Chinese crabs acclimated to fresh water were mounted in a modified Ussing chamber. Active and electrogenic absorption of sodium and chloride were measured as positive amiloride-sensitive and negative Cl--dependent short-circuit currents (INa, ICl), respectively. Both currents were characterized before and after treatment of the ti...

The Slick (Kcnt2) sodium-activated potassium (KNa) channel is a rapidly gating and weakly voltage-dependent and sodium-dependent potassium channel with no clearly defined physiological function. Within the dorsal root ganglia (DRGs), we show Slick channels are exclusively expressed in small-sized and medium-sized calcitonin gene-related peptide (CGRP)-containing DRG neurons, and a pool of chann...

Chronic metabolic acidosis increased the Na+-H+ exchange activity in isolated renal brush-border membrane vesicles. Treatment altered the initial rate of Na+ uptake by increasing Vm (acidotic, 15.3 +/- 0.7 nmol of Na+ X mg-1 X 2 s-1; normal, 11.3 +/- 0.9 nmol of Na+ X mg-1 X 2 s-1), and not the apparent affinity KNa+ (acidotic, 10.2 +/- 0.5 mM; normal 10.2 +/- 0.6 mM). Metabolic acidosis result...

BETZ, A. LORRIS, DAVID D. GILBOE, DAVID L. YUDILEVICH, AND LESTER R. DREWES. Kinefics of unidz’rechzal glucose fransport into the isoluted dog brain. Am. J. Physiol. 225(3): 586592* 1973.An indicator-dilution technique with 22Na as the intravascular marker was used to measure unidirectional transport of D-glucose6-3H from blood into the isolated dog brain. The rate of unidirectional glucose tra...

Familial adenomatous polyposis (FAP) is an inherited disorder caused by germline mutation of the adenomatous polyposis coli (APC) gene. Increased risk of hepatoblastoma (HBL) in FAP kindreds has been re ported. To determine whether inactivation of the APC gene plays a role in development of HBL, 13 sporadic infantile hepatic tumors were analyzed for genetic alterations in the APC gene. A PCR-me...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Materials and media 3.1.1. Preparation of thymocytes 3.2. Mg loading procedure 3.3. Measurement of intracellular Na and Mg 3.4. Measurement of Nao-induced Mg efflux 3.5. Measurement of Nao-induced Mgit and Nait changes 3.6. Statistical analysis 4. Results 4.1. Nait and Mgit content 4.2. Plasmalemmal Mg leak was not enhanced by A-23187 Mg...

The administration of cortisone to rats and rabbits has been found to cause profound changes in the hepatic parenchyma; i.e . . . . increase in cell size, accumulation of glycogen, and greatly decreased cytophsmic basophilia which has been found not to be decreased further by treatment with ribonudease (1-4). Microscopic examination has also indicated a decrease in numbers of mitochondria and a...

The reaction between [XW(9)O(34)](9-) (X = As, P) and [RuC(6)H(6)Cl(2)](2) in aqueous buffer solution (pH 6.0) leads to the isolation of two pseudo-sandwich-type heteropolytungstates KNa(6)[(RuC(6)H(6))AsW(9)O(34)] x 17 H(2)O (As-1) and Na(7)[(RuC(6)H(6))PW(9)O(34)] x 14 H(2)O (P-2). Single-crystal X-ray diffraction analyses reveal that the two compounds have the following features: (1) in two ...

Neurons in the midline and intralaminar thalamic nuclei are components of an interconnected brainstem, limbic and prefrontal cortex neural network that is engaged during arousal, vigilance, motivated and addictive behaviors, and stress. To better understand the cellular mechanisms underlying these functions, here we review some of the recently characterized electrophysiological and neuropharmac...

BACKGROUND Pathogens exert selective pressure which may lead to substantial changes in host immune responses. The human complement receptor type 1 (CR1) is an innate immune recognition glycoprotein that regulates the activation of the complement pathway and removes opsonized immune complexes. CR1 genetic variants in exon 29 have been associated with expression levels, C1q or C3b binding and inc...