The binding behavior of a competitive inhibitor, N-phenylbenzhydroxamic acid
(BHA) against horseradish peroxidase (HRP) was studied in order to understand and
predict the interaction mechanism of hydrogen donors with the enzyme. The
dissociation constants of the complexes of HRP-BHA, HRP-donor and HRP-BHA-azide were estimated at specified conditions by difference spectroscopy. The binding s...
