The vibrational analysis of elastic models suggests that the essential motions of large biomolecular assemblies can be captured efficiently at an intermediate scale without requiring knowledge of the atomic structure. While prior work has established a theoretical foundation for this analysis, we demonstrate here on experimental electron microscopy maps that vibrational modes indeed describe fu...

By using single-molecule fluorescence resonance energy transfer (smFRET), we observe the real-time dynamic coupling between the ribosome, labeled at the L1 stalk, and transfer RNA (tRNA). We find that an interaction between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptide bond formation; this event involves coupled movements of the L1 stalk and tRNA...

Stainless steels with harmonic-structure design have a great balance of high strength and ductility. Therefore, it is imperative to investigate their fatigue properties for engineering applications. In the present work, harmonic-structured SUS316L stainless were fabricated by mechanical milling (MM) subsequent hot isostatic pressing (HIP) process. A series ratcheting-fatigue tests performed on ...

This paper presents a numerical model based on the Lattice Boltzmann Method (LBM), developed for studying dynamic responses of an unsaturated porous medium to periodic imbibition and drainage induced by a cyclic water table movement. The model includes gravity which helps defining an hydraulic head. The model predicted an incremental increase of the overall water content in the medium over each...

Cellular forces generated in the apical domain of epithelial cells reshape tissues. Recent studies highlighted an important role for dynamic actomyosin contractions, called pulses, that change cell and tissue shape. Net cell shape change depends on whether cell shape is stabilized, or ratcheted, between pulses. Whether there are different classes of contractile pulses in wild-type embryos and h...

This paper describes the fabrication of a composite agar/PDMS device for enriching short cells in a population of motile Escherichia coli. The device incorporated ratcheting microchannels, which directed the motion of swimming cells of E. coli through the device, and three sorting junctions, which isolated successively shorter populations of bacteria. The ratcheting microchannels guided cells t...