In this study, we demonstrated that the methyltransferase activity associated with Dam was essential for attenuation of Aeromonas hydrophila virulence. We mutated aspartic acid and tyrosine residues to alanine within the conserved DPPY catalytic motif of Dam and transformed the pBAD/damD/A, pBAD/damY/A, and pBAD/damAhSSU (with the native dam gene) recombinant plasmids into the Escherichia coli ...

The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescens Sr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenin...

The objective of this study was to evaluate the opinions and attitudes of dentists toward the use of rubber dam during various restorative procedures in their dental practices. A structured questionnaire was designed, and distributed to 300 dentists and those that were fully completed were collected for analysis. The collected informations about the opinions and attitudes of dentists toward the...

Susamyia Ziegler & Gilasian gen. nov. and its type species mira et sp. are described from southwestern Karkheh National Park southeastern Jazmourian Wetland of Iran. Drawings terminalia head the male as well images habitus, egg provided. is incorporated into identification key to Palearctic tachinid genera Tschorsnig Richter (1998). The systematic position in tribe Goniini (Exoristinae) dis...

BACKGROUND Dams are important to ensure food security and promote economic development in sub-Saharan Africa. However, a poor understanding of the negative public health consequences from issues such as malaria could affect their intended advantages. This study aims to compare the malaria situation across elevation and proximity to dams. Such information may contribute to better understand how ...

1. introduction one of the main characteristics of azadegan plain in the west of khuzestan province is active instability of geomorphic features of karkheh river this instability caused extensive variations in the sedimentary setup in the western parts of this plain, such as buried and abandoned river beds, which are repeated over time. therefore, these variations should be studied in more deta...

Microsoft SharePoint as a Digital Asset Management (DAM) System Harry Hurt Graphic Communication Department, June, 2010 Advisor: Dr. Harvey Levenson The purpose of this study is to determine to what degree Microsoft SharePoint can be used as a digital asset management (DAM) system. DAM systems are utilized to manage the creation, storage, utilization, and distribution of digital rich media file...

DNA adenine methylase (Dam(-)) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the sigma(70) factor. Regulation of finP transcription by Dam methylation ...

Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. The purpose of this study was to determine the role played by the dam ortholog identified in the entomopathogenic bacterium Photorhabdus luminescens. Complementation assays o...

The Three Gorges Dam, located in the largest endemic area of schistosomiasis in China, is one of the world's largest hydroelectric projects to date. Some large-scale hydro projects have resulted in schistosomiasis emergence or re-emergence. Therefore, the dam's potential impact on the transmission of Schistosoma japonicum has raised concerns from medical researchers worldwide. A systematic lite...