Spirochete periplasmic flagella (PFs), including those from Brachyspira (Serpulina), Spirochaeta, Treponema, and Leptospira spp., have a unique structure. In most spirochete species, the periplasmic flagellar filaments consist of a core of at least three proteins (FlaB1, FlaB2, and FlaB3) and a sheath protein (FlaA). Each of these proteins is encoded by a separate gene. Using Brachyspira hyodys...

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BACKGROUND Rapid molecular diagnostics for detecting multidrug-resistant and extensively drug-resistant tuberculosis (M/XDR-TB) primarily identify mutations in Mycobacterium tuberculosis (Mtb) genes associated with drug resistance. Their accuracy, however, is dependent largely on the strength of the association between a specific mutation and the phenotypic resistance of the isolate with that m...

Oxygenases are promising biocatalysts for performing selective hydroxylations not accessible by chemical methods. Whereas toluene 4-monooxygenase (T4MO) of Pseudomonas mendocina KR1 hydroxylates monosubstituted benzenes at the para position and toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 hydroxylates at the ortho position, toluene 3-monooxygenase (T3MO) of Ralstonia pickettii P...

On the category of bisimplicial sets there are different Quillen closed model structures associated to various definitions of fibrations. In one of them, which is due to Bousfield and Kan and that consists of seeing a bisimplicial set as a simplicial object in the category of simplicial sets, fibrations are those bisimplicial set maps such that each of the induced simplicial set maps is a Kan f...

Bezem, Coquand, and Huber have recently given a constructively valid model of higher type theory in a category of nominal cubical sets satisfying a novel condition, called the uniform Kan condition (UKC), which generalizes the standard cubical Kan condition (as considered by, for example, Williamson in his survey of combinatorial homotopy theory) to admit phantom “additional” dimensions in open...