We investigated the transcriptomic response of a new strain of the yeast Kluyveromyces marxianus, in glucose and xylose media using RNA-seq. The data were explored in a number of innovative ways using a variety of networks types, pathway maps, enrichment statistics, reporter metabolites and a flux simulation model, revealing different aspects of the genome-scale response in an integrative syste...

The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentration...

The present work is initiated to investigate whether a defined culture comprising a mixture of three yeast species, Kluyveromyces marxianus, Saccharomyces cerevisiae, and Pichia stipitis can ferment a mixture of sugars to produce bioethanol at rates higher than those achieved by pure cultures of the same. For this purpose, we develop models of single species based on the hybrid cybernetic model...

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Me...

Background High temperature inhibits cell growth and ethanol fermentation of Saccharomyces cerevisiae. As a complex phenotype, thermotolerance usually involves synergistic actions of many genes, thereby being difficult to engineer. The overexpression of either endogenous or exogenous stress-related transcription factor genes in yeasts was found to be able to improve relevant stress tolerance of...

Here, we present the draft genome sequence of Kluyveromyces marxianus CCT 7735 (UFV-3), including the eight chromosomes and the mitochondrial genomic sequences.

Gaohua Liua,∗, Jeffrey L. Millsa,∗, Tracy A. Hessa, Seho Kima,d, Jack J. Skalickya,e, Dinesh K. Sukumarana, Eriks Kupceb, Arne Skerrac & Thomas Szyperskia,∗∗ aDepartment of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260, U.S.A.; bVarian NMR Systems, Oxfordshire OX8 1JN, U.K.; cLehrstuhl für Biologische Chemie, Technische Universität München, 85350 Freising...

This study aims to identify yeasts from spontaneously fermented herbs used for Van herby cheese and evaluate their enzymatic activities. Sequencing of partial 26S rRNA gene revealed the species Pichia membranifaciens (n = 8), Kazachstania servazzii 6), Debaryomyces hansenii 2), Kluyveromyces marxianus fermentans 2). Enzyme profiles were determined using API-ZYM strips. The isolates had diverse ...