نتایج جستجو برای: jak2v617f

تعداد نتایج: 776  

2014
M.E. Bjørn K. de Stricker L. Kjær K. Ellemann H.C. Hasselbalch

We report a 55 year old woman with post-ET PV for 12 years, who experienced resolution of severe constitutional symptoms within 3 days, a marked reduction in splenomegaly and a rapid decline in the JAK2V617F allele burden during combination therapy with interferon-alpha2a and ruxolitinib. Within 4 weeks the patient achieved complete hematological remission with normalization of peripheral blood...

Journal: :Haematologica 2009
Eric Lippert François Girodon Emma Hammond Jaroslav Jelinek N Scott Reading Boris Fehse Katy Hanlon Mirjam Hermans Céline Richard Sabina Swierczek Valérie Ugo Serge Carillo Véronique Harrivel Christophe Marzac Daniela Pietra Marta Sobas Morgane Mounier Marina Migeon Sian Ellard Nicolaus Kröger Richard Herrmann Josef T Prchal Radek C Skoda Sylvie Hermouet

BACKGROUND Many different techniques have been designed for the quantification of JAK2V617F allelic burden, sometimes producing discrepant results. DESIGN AND METHODS JAK2V617F quantification techniques were compared among 16 centers using 11 assays based on quantitative polymerase chain reaction (with mutation-specific primers or probes, or fluorescent resonance energy transfer/melting curve...

2009
Francesco Dentali Alessandro Squizzato Lorenza Brivio Lorena Appio Leonardo Campiotti Mark Crowther Anna Maria Grandi Walter Ageno

Recent studies suggested that JAK2V617F mutation is frequent inpatientswithsplanchnic vein thrombosis (SVT) but not in patients with other venous thromboembolic events (VTE). However, whether screening for the JAK2V617F mutation in VTE patients is justified remains unclear. Therefore, we performed a systematic review to assess the frequency of JAK2 mutation in VTE patients and the role of JAK2V...

Journal: :Blood 2009
Paola Guglielmelli Giovanni Barosi Giorgina Specchia Alessandro Rambaldi Francesco Lo Coco Elisabetta Antonioli Lisa Pieri Alessandro Pancrazzi Vanessa Ponziani Federica Delaini Giovanni Longo Emanuele Ammatuna Vincenzo Liso Alberto Bosi Tiziano Barbui Alessandro M Vannucchi

A total of 186 patients with primary myelofibrosis (PMF) were genotyped for JAK2V617F at diagnosis aimed at analyzing the correlation of mutational status and mutated allele burden with outcome variables, including time to anemia, leukocytosis, leukopenia, thrombocytopenia, massive splenomegaly, leukemia, and with overall survival. A total of 127 JAK2V617F-mutated patients (68% of whole series)...

Journal: :Blood 2010
Alfonso Quintás-Cardama Kris Vaddi Phillip Liu Taghi Manshouri Jun Li Peggy A Scherle Eian Caulder Xiaoming Wen Yanlong Li Paul Waeltz Mark Rupar Timothy Burn Yvonne Lo Jennifer Kelley Maryanne Covington Stacey Shepard James D Rodgers Patrick Haley Hagop Kantarjian Jordan S Fridman Srdan Verstovsek

Constitutive JAK2 activation in hematopoietic cells by the JAK2V617F mutation recapitulates myeloproliferative neoplasm (MPN) phenotypes in mice, establishing JAK2 inhibition as a potential therapeutic strategy. Although most polycythemia vera patients carry the JAK2V617F mutation, half of those with essential thrombocythemia or primary myelofibrosis do not, suggesting alternative mechanisms fo...

2014
Juan Li David G Kent Anna L Godfrey Harriet Manning Jyoti Nangalia Athar Aziz Edwin Chen Kourosh Saeb-Parsy Juergen Fink Rachel Sneade Tina L Hamilton Dean C Pask Yvonne Silber Xiaodong Zhao Cedric Ghevaert Pentao Liu Anthony R. Green

Genomic regions of acquired uniparental disomy (UPD) are common in malignancy and frequently harbor mutated oncogenes. Homozygosity for such gain-of-function mutations is thought to modulate tumor phenotype, but direct evidence has been elusive. Polycythemia vera (PV) and essential thrombocythemia (ET), two subtypes of myeloproliferative neoplasms, are associated with an identical acquired JAK2...

Journal: :Blood 2013
Samantha F Moore Roger W Hunter Matthew T Harper Joshua S Savage Samreen Siddiq Sarah K Westbury Alastair W Poole Andrew D Mumford Ingeborg Hers

Patients with myeloproliferative disorders (MPDs), such as essential thrombocythemia (ET) have increased risk of thrombosis and bleeding, which are major sources of morbidity and mortality. Most MPD patients have a gain of function mutation in Janus kinase 2 (JAK2V617F), but little is known how JAK2V617F affects platelet function. Here, we demonstrate that platelets from ET patients have impair...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2014
Raajit Rampal Jihae Ahn Omar Abdel-Wahab Michelle Nahas Kai Wang Doron Lipson Geoff A Otto Roman Yelensky Todd Hricik Anna Sophia McKenney Gabriela Chiosis Young Rock Chung Suveg Pandey Marcel R M van den Brink Scott A Armstrong Ahmet Dogan Andrew Intlekofer Taghi Manshouri Christopher Y Park Srdan Verstovsek Franck Rapaport Philip J Stephens Vincent A Miller Ross L Levine

Patients with myeloproliferative neoplasms (MPNs) are at significant, cumulative risk of leukemic transformation to acute myeloid leukemia (AML), which is associated with adverse clinical outcome and resistance to standard AML therapies. We performed genomic profiling of post-MPN AML samples; these studies demonstrate somatic tumor protein 53 (TP53) mutations are common in JAK2V617F-mutant, pos...

2015
Ipek Yonal-Hindilerden Aynur Daglar-Aday Basak Akadam-Teker Ceylan Yilmaz Meliha Nalcaci Akif Selim Yavuz Deniz Sargin

BACKGROUND Despite insights into the genetic basis of Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs), a significant proportion of essential thrombocythemia (ET) and primary myelofibrosis (PMF) patients present with no known MPN disease alleles. There were no previous studies investigating the impact of ASXL1 mutations in Ph-negative MPNs in Turkey. In the current study, w...

Journal: :international journal of hematology-oncology and stem cell research 0
mehrdad payande medical biology research center, kermanshah university of medical sciences, kermanshah, iran mohammad erfan zare medical biology research center, kermanshah university of medical sciences, kermanshah, iran; student saber ghanbari haji shure student of medical lab sciences in research committee, kermanshah university of medical sciences, kermanshah, iran farhad shaveisi zadeh medical genetics department, faculty of medicine, shahid beheshti university of medical sciences and

myeloproliferative neoplasms (mpns) such as polycythemia vera, essential thrombocythemia, primary myelofibrosis and chronic myeloid leukemia have too similar and accurate way to differentiate their is study of genetic disorders in these patients. philadelphia chromosome is a sure way to definitively diagnose cml. recently, jak2v617f mutation introduced as a diagnostic marker for other myeloprol...

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