the botrytis cinerea is one of the most important agents of strawberry fruit rot. in order to identify the pathogenicity potential of the isolates on fruits and the antifungal effect of anise essence on this fungus, forty one isolates of b. cinerea was collected from different areas of mazandaran province on strawberry showing symptoms of gray rot on fruits. following the purification of the fu...

In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia ...

INTRODUCTION Vaginal candidiasis is a condition that affects innumerous fertile women. Candida albicans is the most frequently isolated species from vaginal discharge; however, other different species that are more resistant to antifungal drugs can be identified in vaginal clinical samples. METHODS The species of 30 vaginal Candida isolates was identified by PCR using the universal ITS4 prime...

Specific oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in vivo. Universal fungal primers ITS1 and ITS4, directed to the conserved regions of ribosomal DNA, were used to amplify DNA from Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, Paracoccidioides brasiliensis, Penicillium marneffei, Sporothrix schenckii...

The internal transcribed spacer (ITS) regions of rDNA and the intervening 5.8S rRNA gene for the powdery mildew fungi Erysiphe (sect. Microsphaera) pulchra and Phyllactinia guttata were amplified using standard polymerase chain reaction (PCR) protocols and the universal primer pairs, ITS1 and ITS4. PCR products for ITS were analysed by electrophoresis in a 1.5% agarose gel and sequenced. The si...

Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus ne...

BACKGROUND Recently, identification of fungi have been supplemented by molecular tools, such as ribosomal internal transcribed spacer (ITS) sequence analysis. According to these tools, morphological Exophiala species was newly introduced or redefined. OBJECTIVE This study was designed to investigate the phylogenetics based on ribosomal ITS sequence analysis from clinical Exophiala species iso...

OBJECTIVES To compare two identification methods, i.e., restriction fragment length polymorphism (RFLP)-PCR analysis and enzymatic method Rapid TM Yeast Plus System to identify different species causing vulvovaginal candidiasis (VVC). MATERIALS AND METHODS Vaginal discharges of women who had attended the gynecology outpatient clinic of Mobini Hospital in Sabzevar, Iran were collected using co...

A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were 25℃ and pH 7, respectiv...

In May 2022, a heavily diseased patch of the spring ephemeral, Cardamine concatenata, was observed in Gambrill State Park (39.46425° N, 77.49221° W) Frederick County, Maryland, USA. Symptoms included necrotic patches delimited by veins, with variable chlorosis (Fig. 1). Sparse grey sporulation visible on abaxial leaf surface. Disease incidence 10 × 20 m 100% and severity – individual plants. Mo...