The aim of these studies was to develop a protocol for the isolation and purification pregnancy-associated glycoproteins (PAG) from cotyledons placenta pregnant cows. As result this work, processes parameters PAG were determined, including: extraction in buffer system, two-stage precipitation with ammonium sulfate (40% 80% saturation) target product using ion-exchange chromatography Q Sepharose...

this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...

extraction of intact quality dna from plant tissues, especially those rich in secondary metabolites, is often challenging. literally, hundreds of different dna isolation protocols from various plant species have been published over the last decades. although many commercial dna isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...