Experiments were undertaken to study the effect of introducing isolated mammalian cell mito chondria into tissue culture cells. The D N A of isolated mitochondria was labeled in vitro with 3 Hthymidine. Incorporation of 3H-thymidine into mitochondrial D N A was increased tenfold by the addition of bovine serum albumin and sucrose to the assay. Labeled HeLa cell mitochondria were fused with WI38...

The respiration of isolated peanut (Arachis hypogea) embryos has been studied with dry and wet embryos and mitochondria prepared after various times of imbibition. Dry seeds respire slowly, apparently via a respiratory chain which is deficient in cytochrome c. Cytochrome c-deficient mitochondria have been prepared from the embryos up to 16 hours following imbibition. These mitochondria can meta...

Hypochlorite (HOCl) is one of the most important mediators inflammatory processes. Recent evidence demonstrates that changes in intracellular calcium pool play a significant role damaging effects hypochlorite and other oxidants. Mitochondria are shown to be targets hypochlorite. But little known about mitochondrial HOCl-induced dysfunction. Using isolated rat liver mitochondria, we showed oxida...

Previous studies demonstrated that CSE induces oxidative stress and its consequences on isolated mitochondria obtained from lung, heart and brain which may provide insight into the role of CSE in human health and disease. The present study was carried out to further characterize and compare toxic effect of CSE extract on isolated mitochondria obtained from either a directly contacting tissue (i...

In this study, we investigated the properties of mitochondria to discriminate fresh from frozen-thawed fish fillets. Mitochondria were isolated gilthead seabream fillets and impact freezing was evaluated by measuring permeability mitochondrial membranes. Freezing led permeabilization inner membranes reduced nicotinamide adenine dinucleotide (NADH). The increase in related shock compared physiol...

pioglitazone (pg) is one of thiazolidinediones used for the treatment of type ii diabetes mellitus. some reports of its hepatotoxicity exist, but the mechanism of its hepatotoxicity is not well known. in the present study, the protective effect of some atp suppliers are investigated against mitochondrial toxicity of pg in isolated rat mitochondria. mitochondrial viability was investigated by mt...

To investigate the effects ofellagic acid (EA) on the cytotoxicity, B-lymphocytes isolated from CLL patients and healthy individuals. Flow cytometric assay was used to measure the percentage of apoptosis versus necrosis, intracellular active oxygen radicals (ROS), mitochondrial membrane potential (MMP) and the caspase-3 activity and then mitochondria were isolated from both groups B-lymphocytes...