Key Challenges of Metabolite Separation 1. Metabolite analysis features complex matrices. Samples from plasma and urine are common, but also from in vitro metabolite studies using liver microsomes, possibly causing elevated background signals or isobaric interferences. 2. Enzymatic metabolization often results in a set of isobaric compounds. MS/MS fragmentation can differentiate constitutional ...

Relativistic density functional calculations were applied to study the separation behaviors of the Am(iii) ion from the Eu(iii) ion by diglycolamide (DGA) and nitrilotriacetamide (NTA) ligands in order to understand the difference in the separation mechanism of their reagents. The complexation reaction was modeled on the basis of previous experimental studies. The calculated energies based on s...

When integrated into microfluidic chips, ion-selective nanoporous polymer and solid-state membranes can be used for on-chip pumping, pH actuation, analyte concentration, molecular separation, reactive mixing, and molecular sensing. They offer numerous functionalities and are hence superior to paper-based devices for point-of-care biochips, with only slightly more investment in fabrication and m...

A magnetic nano-composite based on modified styren-maleic anhydride copolymer and Fe3O4 nano-particles was introduced as a new sorbent for solid-phase extraction and preconcentration of trace levels of silver ion from aqueous solutions. The size and morphology of the nano-sorbent were characterized via X-ray diffraction analysis, scanning electron microscopy and Fourier transform infrared spect...

The extraction and separation of copper(II), zinc(II), cobalt(II), and cadmium(II) were investigated. Both copper(II) and zinc(II) formed ammine-complexes, while cadmium(II) and cobalt(II) formed hydroxide precipitates in an ammonia medium. By the addition of sodium dodecylsulfate (SDS), a copper(II) complex formed an ion-pair (copper-ammine-DS), which was extracted into the SDS phase. However,...

Rotating plasmas have the potential to offer unique capabilities for isotope and mass separation. Among the various electric and magnetic field configurations offering mass separation capabilities, rotating plasmas produced through static or oscillating fields are shown to be a leading candidate for tackling the unsolved problem of large-scale plasma separation. The successful development and d...

ion: Expressive power: Separation of concerns:

INTRODUCTION Mobile-phase ion chromatography (MPIC) is a technique that combines ion-pair chromatography with suppressed conductivity detection. Ion-pair chromatography is commonly used in combination with UV detection, in which case it is referred to as reversedphase ion-pair chromatography (RPIPC). MPIC has the advantage of using conductivity detection, which serves as a universal detection m...

In this study, the fermentation broth of recombinant Pichia pastoris strain ncy-2 was studied. After pretreatment, separation, and purification, lysozyme optimized using biofilm ion exchange separation. Finally, dry enzyme powder prepared by concentrating vacuum drying. The removal rate bacterial cells 99.99% when centrifuged at low temperature. optimum conditions were: transmembrane pressure 0...