Most of the studies on yeast invertase have been concerned only with the external enzyme (a mannan protein which is localized in the cell wall), but recently it has been reported that the invertase inside the cytoplasmic membrane is a smaller form. This paper presents a comparative study of the distribution of invertase isozymes in two stains of yeast and, after the selection of the best condit...

Gibberellic acid and sucrose play significant roles in the increases in invertase and growth in Avena stem segments. About 80% of invertase is readily solubilized, whereas the rest is in the cell wall fraction. The levels of both types of invertase change in a similar manner in the response to gibberellic acid and sucrose treatment. The work described here was carried out with only the soluble ...

Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, inhibitor activity decreased during the increase in invertase activity but late...

A number of studies have introduced mutations into the yeast invertase signal peptide, using it as a model system to elucidate features for targeting, translocation and intracellular transport. Using molecular modelling of the invertase signal peptide we have analysed the hydrophobicity potential and the change in dielectric constant of the energy transfer, when the molecule moves from a hydrop...

The amount of carbon transported into storage organs of crop plants to a large degree determines crop yield. The role of sucrose-cleaving enzymes in this process is not clear and it is the main goal of our work to tackle this question. Sucrose cleavage is catalysed either by invertase or sucrose synthase both of which exist in several isoforms with different subcellular locations. Carrot (Daucu...

In the present study, a mutant strain of Saccharomyces cerevisae EMS-42 was used for the biosynthesis of invertase (E.C.3.2.1.26). Both types of invertases i.e., extracellular and intracellular invertase are present in S. cerevisae. An extracellular invertase was purified to homogeneity by two step purification i.e., ammonium sulfate precipitation and DEAE-Sephadex A-50. The enzyme was present ...

Two α-galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28°C were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65°C, respectively. The KM app for ρ-nitrophenyl-α-D-galactopyranos...

1. The relationship of sucrose and water concentration to invertase activity in vivo and in vitro has been studied under the same environmental conditions. 2. The sucroclastic activity of S. cerevisiae cells and of invertase solutions prepared from them reacts to changes in sucrose and water concentration in an identical manner. 3. The invertase contained in living yeast cells is just as freely...

Most of the studies on yeast invertase have been concerned only with the external enzyme (a mannan protein which is localized in the cell wall), but recently it has been reported that the invertase inside the cytoplasmic membrane is a smaller form. This paper presents a comparative study of the distribution of invertase isozymes in two stains of yeast and, after the selection of the best condit...

Effect of several parameters on inhibition of potato (Solanum tuberosum) invertase by its endogenous proteinaceous inhibitor was determined using homogeneous preparations of both proteins. The inhibitor and invertase formed an inactive complex with an observed association rate constant at pH 4.70 and 37 degrees C of 8.82 x 10(2) per molar per second and a dissociation rate constant of 3.3 x 10(...