The Escherichia coli single strand binding (SSB) protein is an essential protein required for DNA replication and involved in recombination and a number of repair processes. It is a stable homotetramer in solution; however the ssb-1 mutation (His-55 to Tyr) destabilizes the tetramer with respect to monomers and this defect seems to explain the observed phenotype (Williams, K. R., Murphy, J. B.,...

Kinetic parameters characterizing the slow structural isomerization observed via metal ion-dependent intrinsic fluorescence quenching of bovine prothrombin Fragment 1 have been determined. From forward and reverse rate constants, an equilibrium constant of approx. 0.25 is calculated. This result is consistent with the hypothesis that there exists, in the absence of metal ions, an equilibrium be...

The kinetics of carbon monoxide (CO) recombination in H93G myoglobin has been studied as a function of temperature in aqueous buffer solution and in 75% glycerol/buffer solutions. H93G adducts with imidazole (Im), 4-methyl imidazole (4-Me Im), 1-methyl imidazole (1-Me Im), and 4-bromoimidazole (4-Br Im) were analyzed by means of three-state and four-state sequential models. Rate constants for t...

The water relations play a fundamental role in seed comprehension of biology. In order to describe the process of drying and the effect on water activity, which controls biological change in storage, a sound knowledge of the relationship between equilibrium moisture content (EMC) and water activity/equilibrium relative humidity (ERH) is essential. The relationship between the total moisture con...

The membrane model previously described [Hays, T.R., Buckwalter, C.Q., Lin S.H. & Eyring, H. (1978) Proc. Natl. Acad. Sci. USA 75, 1612-1615] for ion flow through a membrane is expanded to include the effect of binding of the mobile ion at the occupiable sites in the membrane. Two different effects were investigated: alteration of the association-dissociation rates at constant equilibrium const...