Using a new technique to visualize the tracks of moving 3T3 cells and combining it with the visualization of actin-containing microfilament bundles by indirect immunofluorescence (Lazarides, E. and K. Weber. 1974, Proc. Natl. Acad. Sci. U.S.A. 71:2268-2272), I present experiments which suggest that: (a) 30-40% of the pairs of daughter 3T3 mouse fibroblasts in noncloned cultures have mirror sym...

Twenty-two temperature-sensitive cell cycle mutants of the fungus Aspergillus nidulans, which block in interphase at restrictive temperature, were analyzed by the reciprocal shift method of Jarvik and Botstein (Proc. Nath Acad. Sci. U.S.A. 70:2046-2050, 1973) and Hereford and Hartwell (J. Mol. Biol. 84:445-461, 1974) to determine whether these mutations were blocked at the G1, S, or G2 phase of...

Histone H1 phosphorylation affects chromatin condensation and function, but little is known about how specific phosphorylations impact the function of H1 variants in higher eukaryotes. In this study, we show that specific sites in H1.2 and H1.4 of human cells are phosphorylated only during mitosis or during both mitosis and interphase. Antisera generated to individual H1.2/H1.4 interphase phosp...

A nanoscale representative volume element has been developed to investigate the effect of interphase geometry and property on the mechanical behavior of silica/epoxy resin nanocomposites. The role of interphase–matrix bonding was also examined. Results suggested that interphase modulus and interfacial bonding conditions had significant influence on the effective stiffness of nanocomposites, whi...

A 32P-labeling strategy was developed to study the interaction(s) in tissue culture cells between proteins and nucleic acids. Interphase and mitotic nuclear lamins were studied in Drosophila Kc cells. After bromodeoxyuridine incorporation and in vivo photo-crosslinking with 366 nm light, it was found that interphase lamins were associated with nucleic acid. Interactions with DNA as well as RNA ...

Multiple centrosomes in tumor cells create the potential for multipolar divisions that can lead to aneuploidy and cell death. Nevertheless, many cancer cells successfully divide because of mechanisms that suppress multipolar mitoses. A genome-wide RNAi screen in Drosophila S2 cells and a secondary analysis in cancer cells defined mechanisms that suppress multipolar mitoses. In addition to prote...