A procedure has been described for the purification of nucleoside diphosphatase from bovine liver microsomes. The purified enzyme is shown, by analysis in the ultracentrifuge and by polyacrylamide disc gel electrophoresis, to be nearly homogeneous. The enzyme has an s20,u, of 4.9 S. Thiamine pyrophosphatase was purified together with nucleoside diphosphatase. Evidence has been obtained which in...

1. Evidence has been produced for the formation of 5-phosphomevalonate from potassium dl-mevalonate by the latex of Hevea brasiliensis and by reconstituted freeze-dried serum obtained from this latex. 2. The enzyme, mevalonate kinase, catalysing the formation of 5-phosphomevalonate from potassium dl-mevalonate and ATP has been partially purified. 3. 5-Phosphomevalonate formed by the purified me...

The isometric tension response of single "skinned' rabbit soleus muscle fibers to MgATP and McITP in the absence of calcium was studied. [MgATP] or [MgITP] was varied in solutions of ionic strength 0.30 and temperature 20 degrees C. Steady-state tension that developed in MgATP or MgITP solutions was a biphasic bell-shaped function of log [MgATP] or log [MgITP] which increased from zero to maxim...

It has long been accepted that M g A T P is the energy source for contraction. Evidence has also accumulated indicating that M g A T P plays an important role in the regulation of contraction (Weber and Murray, 1973; Inoue and Tonomura , 1974; Godt, 1974; Orentlicher et al., 1977). In an a t tempt to better understand the dual role of ATP in contraction, several researchers have studied the eff...

Tumor Immunotherapeutic Gels In article number 2204273, Dunfang Zhang, Hang Zhao, and co-workers demonstrate that an inosine-based supramolecular hydrogel, IPBisoG, can achieve the gradual sequential release of inosine aPDL1. Inosine, which enhancs proliferation function CD8+ T cells, together with aPDL1, blocker immunosuppressive pathway in tumor microenvironment, could highly enhance vivo eff...

The distribution of the Mg-dependent ATPase associated with a microsomal fraction of rabbit psoas muscle was studied histochemically and its localization in relation to the vesicles of the fraction and to the structure of intact fixed muscle was determined. Although enzyme activity was retained after fixation in hydroxyadipaldehyde and in glyoxal, it was lost after fixation in glutaraldehyde or...

In the development of the mature rabbit erythrocyte from the reticulocyte, the capacity for the synthesis de novo of the purine ring in vitro is lost (2, 3). The mature rabbit erythrocyte, however, does retain a portion of the biosynthetic pathway de novo, as evidenced by its utilization of 5-amino-1-ribosylWimidazolecarboxamide and sodium formate-Cl4 for the formation of inosinic acid (4). The...

In the NRQCD factorization for productions and decays of a quakonium the difference between the quarkonium mass and the twice of the heavy quark mass is neglected. We attempt to interpret the difference in term of NRQCD matrix elements, and consider the effect introduced by the difference in quarkonium decays. The effect is significant in these decays. Similar effect in decays of heavy-flavor h...

I. MOCIOIU∗, M.POSPELOV R. ROIBAN∗% ∗C.N.Yang ITP SUNY Stony Brook, NY 11794-3840 E-mail:mocioiu, roiban@insti.physics.sunysb.edu Theoretical Physics Institute, School of Physics and Astronomy, University of Minnesota, 116 Church St., Minneapolis, MN 55455 E-mail:pospelov@mnhepw.hep.umn.edu $ McGill University, 3600 University Street, Montreal, Quebec, Canada H3A 2T8 Pysics Department, Universi...