Nuclease activity was dissociated from proteolytic activity when Sachs confirmed the observations of Milroy that an agent which attacked nucleic acid to liberate inorganic phosphoric acid could not liquefy gelatin and that proteolytic enzymes liquefied gelatin but could not attack nucleic acid (1). Henceforth the rate of separation of inorganic phosphoric acid from nucleic acid became the exclu...

Nowadays, proton exchange membrane fuel cells (PEMFCs) are the most promising green energy conversion devices for portable and stationary applications. Traditionally, these devices were based onperfluoro-sulfonic acid electrolytes membranes, given the commercial name Nafion. Nafion is the mostused electrolyte membrane till now; because of its high electrochemical properties su...

By T. J. GREENWALT AND V. E. AYERS T HE DISTRIBUTION of phosphate compounds in acid extracts of the erythrocytes of infants has heretofore been investigated only by fractional analysis for inorganic, total and hydrolyzable phosphates.1 In our previous work hydrolysis of trichloracetic acid extracts of cord blood erythrocytes was used to characterize the following fractions : ( 1 ) labile phosph...

the electrochemical behaviour of lead electrode in hydrochloride solution containing variousoxo-anions at different concentrations was studied by using of cyclic voltammetry (cv) method.results obtained show that the addition of these inorganic compounds decreases the anodic andcathodic currents peaks according to the following order: h2po4- > hpo42-> so42-. it’s revealedalso that the cathodic ...

Inorganic capillary electrophoresis (ICE) is a new separations technology which melds the technique of classical electrophoresis with the separations approach of ion chromatography. Matrices which have been difficult to deal with using ion chromatography have proven amenable to analysis by ICE. The simultaneous analysis of weak acid anions, oxalate and citrate and inorganic anions, chloride, su...

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a ...

Bile acid sulfation was studied in primary hepatocyte cultures. The primary hepatocyte cultures formed glycolithocholate-3-sulfate (GLC-S) when glycolithocholate (GLC) was added to the medium. The relative percentage of GLC-S formation increased when the GLC concentration was increased from 10 microM to 100 microM. GLC-S formation was linear to 60 min. GLC-S secretion into the medium was detect...