A recombinant vaccinia virus inducibly expressing ORF A1 of infectious bursal disease virus (IBDV) has been constructed and characterized. Cells infected with this recombinant virus express the IBDV polyprotein, which is proteolytically processed to give mature VP2, VP3, and VP4 polypeptides. An electron microscopy study revealed that the cytoplasm of cells infected with the recombinant virus c...

The interaction between antibodies and the surface antigens of infectious bursal disease virus particles was studied in the agarose gel by using single radial hemolysis (SRH) test. Sonicated antigen was used to sensitize the Human "O" erythrocytes. Hemolytic zones, using the filter paper discs were observed after overnight incubation in humid chamber at 37C. Indirect Hemagglutination (IHA) and ...

The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV challenge in specificpathogen-free (SPF) chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However, only four ...

Sera from nine out of 30 (30.0%) apparently healthy unvaccinated indigenous (Tswana) chickens had precipitating antibodies to infectious bursal disease (IBD) virus using the agar gel precipitation test. Similarly, sera from 11 out of 49 (22.4%) chickens of exotic breeds with no history of vaccination against IBD were positive for antibodies against the virus.