A cluster of unlinked genes encoding gluconeogenic enzymes in the mouse is characterized by the failure of normal hormone-inducible expression in animals homozygous for one of several overlapping deletions mapping on chromosome 7 near the albino locus. Previous investigations have shown hormones and their receptors to be normal in the mutants and therefore not responsible for the abnormalities ...

In cancer, the use of microbots based on anaerobic bacteria as specific transporters targeting tumor tissues has been explored since most solid tumors exhibit hypoxic regions. The aim this study was to develop and characterize magnetic Bifidobacteria iron oxide fluorescent nanoparticles complexed with chitosan a hypoxia inducible plasmid. addition, efficiency for gene delivery evaluated in an v...

Abstract The transcription factor GATA3 expression is essential for T helper-2 (Th2) cells and type 2 innate lymphoid (ILC2s) mediated immune responses. However, most of the studies were done in vitro system, such functions cannot be easily verified vivo because markers identifying Th2 ILC2s depends on GATA3. We have generated a novel reporter mice strain carrying Gata3 ZsG-flallele. This mouse...

Abiotic stresses such as salinity influence agricultural production. Plants generally respond to stimulus conditions in a complex manner involving many genes and proteins. In the evolution process, halophyte plant Aeluropus littoralis has been proven to have abiotic stress-tolerance capacity. A. littoralis is a salt-resistant halophyte providing a wealthy genetic resource for developing salinit...

Cross-protection of acid-adapted Escherichia coli O157:H7 against inimical stresses is mediated by the glucose-repressed sigma factor RpoS. However, many food systems in which E. coli O157:H7 occurs are complex and contain glucose. This study was aimed at investigating the contribution of acid and lactoperoxidase (LP)-inducible genes to cross-protection of E. coli O157:H7 against LP system and ...

Generation of multiple genomic alterations is currently a time consuming process. Here, a method was established that enables highly efficient and simultaneous deletion of multiple genes in Escherichia coli. A temperature sensitive plasmid containing arabinose inducible lambda Red recombineering genes and a rhamnose inducible flippase recombinase was constructed to facilitate fast marker-free d...

Class II transcriptional activator (CIITA) is a master regulator of MHC class II genes, including DR, DP, and DQ, and MHC class II-associated genes DM and invariant chain. To determine the repertoire of genes that is regulated by CIITA and to identify uncharacterized CIITA-inducible genes, we used representational difference analysis. Representational difference analysis screens for differentia...

Ubiquitous isethionate (2-hydroxyethanesulfonate) is dissimilated by diverse bacteria. Growth of Cupriavidus necator H16 with isethionate was observed, as was inducible membrane-bound isethionate dehydrogenase (IseJ) and inducible transcription of the genes predicted to encode IseJ and a transporter (IseU). Biodiversity in isethionate transport genes was observed and investigated by transcripti...