Thermostabilizing effect of heavy water (D2O) or deuterium oxide has been demonstrated previously on several enzymes and vaccines like oral poliovirus vaccine and influenza virus vaccine. In view of the above observations, effect of heavy water on in situ thermostabilization of recombinant p26 protein on enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of equine infectious anemia vir...

The avidin-biotin enzyme-linked immunosorbent assay (A-B ELISA), for use in surveillance for bovine brucellosis in India was developed and calibrated using the indirect brucellosis ELISA kit of the International Atomic Energy Agency (IAEA) as a reference. The reagents used in the A-B ELISA were as follows: the smooth lipopolysaccharide of Brucella abortus strain 99 (antigen); biotinylated anti-...

For serological investigation of N. caninum infection in dogs more tests are available, including indirect fluorescent antibody test (IFAT), often considered as the reference test, and enzyme-linked immunosorbent assay (ELISA). A total of 28 dogs were screened with a commercially multi-species indirect ELISA, including a subset of 9 samples previously tested by IFAT 1:50. A partial correlation ...

OBJECTIVE To investigate the diagnostic value of commercially available BP230 and BP180-NC16a enzyme-linked immunosorbent assays (ELISAs) in routine practice in patients with bullous pemphigoid (BP). DESIGN Single-center retrospective study. SETTING French academic dermatology department. PATIENTS The study population comprised 138 patients, who were admitted from January 1998 through Dec...

Hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. Clinical surveillance for Hantaan virus infection is unreliable, and laboratory verification is essential. The detection of virus-specific immunoglobulin M (IgM) and IgG in serum is most commonly used for the diagnosis of hantavirus infection. Testing of oral fluid samples instead of serum offers many advantag...

in the present study, seven isolates of haemophilus influenzae type b were cultured in four different media to compare growth rate and capsule production. four liquid media namely brain heart infusion broth (bhi), trypticase soy broth (tsb), mueller hinton broth (mhb) and gonococci broth (gc) with added supplements (1% hemoglobin, 1% isovitalex) were used. the growth was measured by colony coun...

An enzyme-linked immunosorbent assay (ELISA) test has been developed to detect antibodies to Legionella pneumophila serogroup 1. There is good correlation between indirect fluorescent antibody (IFA) and ELISA titres but ELISA is more sensitive.

the HI assay can be influenced by non-specific serum inhibitors the reactivity of both HI and SN assays strongly depends on the test virus strain used (homologous or heterologous strains) 1 . HI titers show a peak 2-3 weeks after experimental infection. After a maximum of 22 weeks, these titers decrease and become negative, while ELISA still may give positive resultats 3 . Indirect ELISAs seem ...

The sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. Tests evaluated included the U.S. Department of Agriculture (USDA) card test with 8% cell concentration (8%Card), USDA rapid automated presumptive test (RAP), Mexican rose bengal plate tests with 8 and 3% cell concentrations (8%RB and 3%RB), French rose bengal plate test with 4.5% cell co...