نتایج جستجو برای: indirect immunofluorescence

تعداد نتایج: 102331  

2012
Jörn Voigt Christopher Krause Edda Rohwäder Sandra Saschenbrecker Melanie Hahn Maick Danckwardt Christian Feirer Konstantin Ens Kai Fechner Erhardt Barth Thomas Martinetz Winfried Stöcker

Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF inc...

Journal: :Clinical chemistry 2004
Xavier Bossuyt Johan Frans Ann Hendrickx Godelieve Godefridis René Westhovens Godelieve Mariën

BACKGROUND HEp-2 cells that overexpress the human 60-kDa SSA antigen have been used to improve sensitivity and specificity for the detection of anti-SSA antibodies by indirect immunofluorescence. We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA. METHODS The evaluation was done on 18 371 consecutive samples submitted to the labora...

Journal: :Arthritis Research & Therapy 2009
Michael Mahler Jennifer T Ngo Johannes Schulte-Pelkum Tanja Luettich Marvin J Fritzler

Reference 1. Mahler M, Ngo JT, Schulte-Pelkum J, Luettich T, Fritzler MJ: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies. Arthritis Res Ther 2008, 10:R131. Correction Correction: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies Michael Mahler1, Jennifer T Ngo2, Johannes Schult...

Journal: :Journal of clinical microbiology 1980
L Mintz R C Miner A S Yeager

Cytomegalovirus antibodies were measured in human sera by a nuclear anticomplement immunofluorescence test that used as antigen the isolated nucleic of virus-infected fibroblasts cells lysed in distilled water. The method exhibited less nonspecific fluorescence than either a conventional whole-cell anticomplement immunofluorescence test or an indirect fluorescent antibody test applied to the sa...

Journal: :iranian red crescent medical journal 0
nima mirzaei department of biology, science and research branch, islamic azad university, tehran, ir iran; department of biology, science and research branch, islamic azad university, tehran, ir iran. tel: +98-2188962343 talat mokhtari azad department of virology, tehran university of medical sciences, tehran, ir iran rakhshandeh nategh department of virology, tehran university of medical sciences, tehran, ir iran hoorieh soleimanjahi department of virology, tarbiat modares university, tehran, ir iran nour amirmozafari department of microbiology, tehran university of medical sciences, tehran, ir iran

background: sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. ectodomain of influenza m2 protein is a surface and highly conserved protein. m2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. objectives: in this study, a recombinant baculovirus containing m2e and cholera toxin subunit b fus...

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