S EVERAL RECENT REPORTS have incriminated reticulocytes as the cause of “false positive” Coombs tests obtained in spontaneous or experimentally induced anemia.14 As reticulocytosis is frequently associated with the spontaneous Coombs positive hemolytic anemia which develops in NZB mice, and because the positive Coombs test has been used by Holmes and Burnet5T as the main basis for calling the c...

Out of 177 Rh(D)-negative patients studied, 96 were successfully followed-up after spontaneous or therapeutic abortion. Rh antibodies were detected by the indirect Coombs test in two patients and by an enzyme technique only in a further seven, an overall incidence of 9.4%.The prophylactic use of anti-D immunoglobulin is now recommended for Rh-negative non-immunized patients undergoing abortion,...

STATEMENT OF PROBLEM Patterns for custom cast posts and cores can be fabricated either by the direct or the indirect technique. Which technique is more accurate is unknown. PURPOSE The purpose of this in vitro study was to investigate the effect of pattern fabrication technique on the accuracy of post fit. MATERIAL AND METHODS Ten intact extracted premolar teeth with a single canal and simi...

BACKGROUND The INDIAN blood group system comprises 4 antigens sensitive to enzymes and 2-aminoethyl isothiouronium bromide (AET). AIM The patient's antibody was investigated for its specificity to the high-frequency antigens (HFA) of this system. MATERIAL AND METHODS Low ionic strength solution (LISS)-tube/LISS-indirect antiglobulin test (IAT) methods were used. The patient's red blood cell...

SYNOPSIS Erythrocytes may be coated with blood group antibodies with or without reacting complement or sometimes apparently with complement alone. This may occur in vivo in such conditions as autoimmune acquired haemolytic anaemia, haemolytic disease of the newborn, or after transfusions of incompatible blood. It may occur in vitro also by the deliberate sensitization of erythrocytes during lab...

During a phase I evaluation of diglycoaldehyde (INOX), an intravenous chemotherapeutic agent used to treat children with malignancies, all of eight patients tested developed a positive direct antiglobulin test (DAT) in vivo. The DAT became positive within one to seven days after the first administration of the drug and remained positive for up to 12 days following the last dose. The indirect an...

In the present study, direct antiglobulin test (DAT) was performed using poly-antiglobulin serum on a total of 138 individuals; 20 of whom had HIV infection only, 50 malaria only, 18 both malaria and HIV and 50 non-infected with either malaria or HIV in order to determine the effect of co-infection with malaria and HIV on DAT status. Malaria parasite test was done by microscopic examination of ...

We evaluated the validity and the usefulness of a new test for the diagnosis of human brucellosis based on an immunocapture-agglutination technique. A total of 315 sera from 82 patients with a diagnosis of brucellosis, 157 sera from patients in whom brucellosis was suspected but not confirmed, and 412 sera from people living in rural areas with endemic brucellosis were studied. The seroagglutin...

This study sought to elucidate the mechanism by which human red cells, in a variety of clinical settings, become coated in vivo with autologous complement components in the absence of anti-red cell autoantibodies demonstrable by standard methods. By means of a newly developed complement-fixing antibody consumption test, previously undetectable red cell-bound gammaG globulin could be detected an...