in the present study we aimed to evaluate the effects of methanolic extracts of the bulbs of garlic (allium sativum l., alliaceae) and persian shallot (allium ascalonicum l., alliaceae ) and leaves of sage (salvia officinalis l., lamiaceae ), ase, aae and soe respectively, on the antioxidant enzymes such as superoxide dismutase (sod), glutathione peroxidase (gpx) and catalase (cat) activities an...

A study of the optimization of CuZn superoxide dismutase gene expression in E. coli L21/pET28-SOD was undertaken. The SOD gene was directionally cloned into expression vector pET28.The recombinant plasmid was transformed into E. coli BL21 and induced by IPTG to express SOD protein. The four effective factors, including the initial concentration of BL21/pET28-SOD, concentration of IPTG, incubati...

PURPOSE The human cornea, a tissue much exposed to oxidative stress, is rich in extracellular superoxide dismutase (SOD). In this study, the contents and distributions of the SOD isoenzymes in the normal human cornea were compared with those in corneas affected by keratoconus and bullous keratopathy. METHODS The central and peripheral parts of normal human corneas were analyzed separately. Ce...

The prognostic potential of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was evaluated in sepsis. Enzyme concentrations were determined in samples obtained from septic patients at time of diagnosis. Statistically significant increases in activities of total plasma SOD (P < 0.003, n = 32), erythrocyte (RBC) SOD (P < 0.007, n = 16), plasma CAT (P < 0.0001, n = 32), and RB...

withania somnifera (l.) dunal is an erect evergreen shrub commonly known as ashwagandha. it is widely used in ayurvedic, and in the traditional pharmacopeia system of india. it is one of the major ingredients in many formulations prescribed for a variety of musculo-skeletal conditions including arthritis and rheumatism. in the present study, variations in the quality and quantity of proteins an...