data collected confirmed the RNA-Seq results. ChIP-Seq methods are being optimized to identify the direct targets of OpaR. Select targets were confirmed direct targets via in vitro electrophoretic mobility shift assays (EMSA) with purified hexahistidine tagged OpaR. A previously published position specific weighted matrix (PSWM) will be utilized to assist in identification of possible OpaR bind...

The mutL gene product is part of the dam-directed mismatch repair system of Escherichia coli but has no known enzymatic function. It forms a complex on heteroduplex DNA with the mismatch recognition MutS protein and with MutH, which has latent endonuclease activity. An N-terminal hexahistidine-tagged MutL was constructed which was active in vivo. As a first stop to determine the functional doma...

in the second half of the nineteenth century realistic stories were replaced by fictive ones in urdu literature.wisdom and the important purposes of the aligarh movement made very big changes in the fiction and novel. nazir ahmad and abul-halim sharar were the first authors who created the novel. their traditional beliefs could be felt in their novels. in their books and stories, first they wer...

gravity's rainbow is among the “most widely celebrated, unread novels” of american literature and already “a piece of minor folklore.” pynchon's genius manifests itself in his uniquely wide range of subject matter and literary techniques of presentation, narration, and interpretation. gravity's rainbow is a novel based on various sets of parallels, oppositions and double structures. these paral...

BACKGROUND Silas Weir Mitchell (1829 to 1914), one of the most important neurologists in American Medicine, was known for his seminal work on the phantom limb syndrome, causalgia, and nerve injuries. He was also a prolific writer of novels and short stories. The neurologic content of this fiction has not been studied. OBJECTIVE To assess the extent that references to neurologic topics were pr...

This study focuses on Arashi ha Mujyō Monogatari (“The Tale of Transient Popular Kabuki Actor Arashi’s Life”; 1688), a novel from the early modern Japanese literature, written by Saikaku Ihara (1642–93). It is a first work of a Kabuki actor’s life in Japan (Kabuki is a traditional stage arts performed exclusively by male actors with the accompaniment of live music and songs). Then we will exami...

Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.