نتایج جستجو برای: in his first step

تعداد نتایج: 17114144  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علوم بهزیستی و توانبخشی - دانشکده توانبخشی 1393

abstract objectives gradual increase length and complexity of utterance (gilcu) therapy method is a form of operant conditioning. this type of treatment is very precise and controlled that is done in 54 steps in 3 speech situations consisted of monologue, reading and conversation. this study aimed to examine the effects of gilcu treatment method on reduction of speech dysfluency of school-age...

Journal: :Journal of bacteriology 1995
R Rivera-León C J Green B S Vold

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2007
Christina T Hauser Roger Y Tsien

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

2014
Vida Rodríguez Juan A Asenjo Barbara A Andrews

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Journal: :Journal of materials chemistry 2012
Nancy Hom Kinjal R Mehta Tsengming Chou Amy B Foraker Frances M Brodsky Kent Kirshenbaum Jin K Montclare

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده ادبیات و علوم انسانی 1391

peace and national security protection and ironic solving of international conflicts become the preliminary foundation of the united nations charter and had been considered as the main responsibilities of the security council, after world war ii and the establishment of united nations organization. therefore, the security council enables to analyze every kind of conflicts and challenges which ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد 1376

in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد مرودشت - دانشکده علوم پایه 1393

the present study attempts to discover ideological differences between alcott’s novel (1868) named “little women” and its two persian translations. the first has been translated by morvarid and the second has been translated by obeidi. to this end, farahzad’s cda approach (2012) was used to analyze the ideology reflected in both translations by the two different translators. according to farahz...

Journal: :Applied and environmental microbiology 2006
Andrea Campisano Christine Schroeder Mirle Schemionek Joerg Overhage Bernd H A Rehm

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده علوم انسانی 1390

this study was designed and conducted to find out if there is any significant relationship between iranian efl learners preferance for one of the three degrees of obtrusiveness in foreign language instruction (focus on form, forms and meaning) and their preferred experiential learing style. to collect the data two questionnaires were used. the first one on the degree of obtrusiveness and the se...

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