Using the technique of immune precipitation of [35S]methionine-labelled infected cell polypeptides followed by SDS-polyacrylamide gel electrophoresis and autoradiography it has been shown that SV5 and a closely related isolate are both antigenically related to human parainfluenza virus type 2. Limited cross-reactions were also demonstrated between parainfluenza virus types 1 and 3 by this metho...

Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...

Cell lysis with mild detergent is commonly used with cultured animal cells. If low detergent concentrations are sufficient to cause cell lysis (e.g., 1% Nonidet P-40 [NP-40] or 1% Triton X-100), this method can be gentler to the protein of interest than mechanical homogenization methods. The choice of detergent must be tailored to the nature of the epitope recognized by the immunoprecipitating ...