We have developed a simple assay for the detection of peripheral blood natural killer cells (NK) metabolic activity based on tetrazolium reduction reaction after the cultivation of isolated NK cells with sperm cells. We have adapted a reliable, inexpensive and easy to prepare method in conjunction with the EZ4U system, while target NK cells' isolation was solved using Dynabeads immunomagnetic t...

The functional antithrombin III assay of von Kaulla is described and modified for the fibrometer with substitution of readily available Owren's buffer for previously described buffers. The assay is compared with immunological techniques on 50 hospital patients and comparable discrimination of normals and patients with thrombotic disease are shown. Normal values are 51.1+/-13.9 secs. Results in ...

Formaldehyde or gluteraldehyde cells treated with tannic acid were coated with plasminogen. These cells are agglutinated by plasminogen antiserum and this reaction can be quantitatively inhibited by purified plasminogen, plasma, and euglobulin. Correlation between this haemagglutination assay and a caseinolytic method is good. The sensitivity of the technique is high and compares favourably wit...

irritable bowel syndrome (ibs) is the most prevalent functional gastrointestinal disorder noted in the general population worldwide. its chronic nature, signs and symptoms which vary periodically from mild to severe have many negative effects on the quality of life for the sufferer; therefore the appropriate treatment of these patients is highly important. patients should be informed by their d...

BK polyomavirus (BKPyV) was isolated in 1971 from the urine of a kidney transplant patient. Soon after its identification, BKPyV was characterized as a kidney-tropic virus, which is responsible of a significant fraction of the rejection of transplant kidney in the host. Moreover, in experimental conditions, BKPyV is able to transform different types of animal and human cells and to induce tumor...

The study aimed to show the effect of diet in observing some immunological and physiological aspects human body. is divided into two parts; first part includes markers that involve interleukins (IL1α, IL 17,) high-sensitivity C-reactive protein (hs-CRP). second biochemical markers; Vitamin D, Total cholesterol, Triglyceride, HDL, LDL, Calcium. These parameters were measured blood from 120 volun...

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA s...

Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae were identified by enzymatic assay. In leaves of three genera, Solanum, Lycop...

A defined immunological cross-reaction was observed between acetylcholine receptor fraction from the electric eel, Electrophorus electricus, and two calf thymus fractions. The cross-reaction was demonstrated on the cellular level by means of the lymphocyte transformation technique, and on the humoral level, by means of the microcomplement fixation assay. In the human disease myasthenia gravis b...