Immunofluorescence techniques and other immunopathologic diagnostic techniques in dermatology involve the detection and localization of specific autoantibodies against various antigens within the skin. Since the 1960s when the lupus band test was utilized to diagnose lupus erythematosus, diagnostic immunopathologic techniques, when interpreted alongside clinical and histopathologic findings, ha...

coccidian protozoa of cyclospora cayetanensis are obligate intracellular apicomplexan parasites that infect the mucosal epithelium of the small intestine of immunocompetent and immunocompromised persons. a 25- years old woman from around, tehran with complaint of faintness and fatigue with hiv positive/aids confirmed eight months ago was admitted in imam khomeini hospital, tehran, iran in 2014....

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

The aim of this study was to investigate the activity apoptosis and autophagy in animals (cows, horses, donkeys, dogs cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, qPCR. mRNA transcript levels caspase-3, Bax, Bcl2 LC3B were determined Caspase-3 AIF immunopositivity not observed immunohistochemical immunofluorescence staining, whereas intensively compare...

We examined the morphological changes in mitochondria, mitochondrial nucleoids (mt-nucleoids), and actin cytoskeleton yeast Saccharomyces cerevisiae during transition from anaerobic to aerobic culture by immunofluorescence microscopy, DAPI staining, rhodamine-phalloidin staining. The cells grown anaerobically stationary phase contained a small number of 7.3 per cell on average. When were transf...

The results of immunofluorescence studies in nine cases of rheumatoid arthritis and nine cases of ankylosing spondylitis are presented. Antiglobulin activity was sought with fluorescein labelled, heat aggregated human IgG and rabbit immunoglobulin (indirect immunofluorescence). A similar degree of increased staining for rheumatoid factor activity was seen after pepsin digestion in all cases of ...

Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DM...