BACKGROUND Gender-related differences in incidence of arterial thrombosis have been a focus of interest for years. The platelet integrin alphaIIbbeta3 is primarily responsible for the interaction between platelets and fibrinogen and consecutive thrombus growth. In this study, we evaluated platelet adhesion onto immobilized fibrinogen under venous and arterial flow conditions in men and women. ...

A rapid pretreatment-free immunochromatographic assay was developed for the control of the streptomycin (STR) content in milk and dairy products. The assay is based on the competition between an immobilized STR-protein conjugate and STR in a sample to be tested for the binding to monoclonal anti-STR antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip wit...

We have recently developed a simple, reusable and coupled whole-cell biocatalytic system with the capability of cofactor regeneration and biocatalyst immobilization for improved production yield and sustained synthesis. Described herewith is the experimental procedure for the development of such a system consisting of two E. coli strains that express functionally complementary enzymes. Together...

A nanostructured biocatalytic interface reversibly gating electrochemical reactions upon chemical signals processed by immobilized enzymes was architectured. The chemical signals were converted to local interfacial pH changes causing restructuring of the stimuli-responsive polymer and switching ON-OFF the electrochemical reaction.

www.MaterialsViews.com C O M M U Nanoporous Scaffold with Immobilized Enzymes during Flow-Induced Gelation for Sensitive H 2 O 2 Biosensing N IC A By Donglai Lu , Joshua Cardiel , Guozhong Cao , and Amy Q. Shen * IO N Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications, while nanoporous scaffolds hold an enormous potential in improving the perform...

A novel enzyme-immobilized flow-through interface was designed for sensitive end-column chemiluminescent (CL) detection in open-tubular capillary electrochromatography (OTCEC). Enzyme was covalently bound on an aldehyde-activated polymer membrane immobilized in a flow cell to catalyze the CL reaction that occurred in it. Using glycine as the model analyte, N-(4-aminobutyl)-N-ethylisoluminol-der...

AIMS To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in binding to immobilized fibronectin and a strong reduction in the surface hydro...

in the present study, streptomyces gulbargensis and its mutant form, s.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of l-asparaginase using groundnut cake extract as medium. the medium with an initial ph of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oc and shaking at 200 rev/min....

lipase from candida antarctica, fixed on macroporous acrylic resin, has been used for the intermediate production of mono- and diolein by hydrolysis of triolein. the effect of altering concentrations of triolein and glycerol and the function of the molecular sieve on the hydrolysis reaction of triolein were investigated. the highest hydrolysis yield was observed for the utmost concentration of ...