A bacterial strain Pseudomonas sp. a3 capable of degrading nitrobenzene, phenol, aniline, and other aromatics was isolated and characterized. When nitrobenzene was degraded, the release of NH(4) (+) was detected, but not of NO(2) (-). This result implied that nitrobenzene might have a partial reductive metabolic pathway in strain a3. However, aniline appeared as one of the metabolites during th...

A quantitative test to study Listeria and Salmonella adherence to epithelial cells was developed. The quantitative test is rapid and allows the simultaneous testing of many variables such as the adherence ability of different bacteria to their target cells as well as the capability of various molecules to inhibit bacterial adherence. By using a strain-specific standard curve in each test, the t...

Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater eth...

This study assesses the ability of mycelia of Rhizopus delemar (both free and immobilized on polyurethane foam) to remove heavy metals from single-ion solutions as well as from a mixture of them. All experiments were conducted using 0.5-5 mm solutions of CuSO4 x 5H2O, CoCl2-6H2O and FeSO4 7H2O. Mycelia immobilized on polyurethane foam cells showed some times increase in uptake compared with tha...

Establishing a cost and time efficient approach for bioprocess optimization is desired but is challenging. In the present work, we have addressed the effectiveness of using immobilized cells for aerobic processes, behaviour of immobilized cells, optimization and upstream bioprocess analysis for the production of lysine by immobilized cells of Corynebacterium glutamicum ATCC 13032 and MH 20-22 B...

BACKGROUND The bacterium Acetobacter sp. CCTCC M209061 is a promising whole-cell biocatalyst with exclusive anti-Prelog stereoselectivity for the reduction of prochiral ketones that can be used to make valuable chiral alcohols such as (R)-4-(trimethylsilyl)-3-butyn-2-ol. Although it has promising catalytic properties, its stability and reusability are relatively poor compared to other biocataly...

Human red blood cells were immobilized in an alginate network which was cross-linked with Ca2+ ions. The immobilized cells were stored for longer than 5 weeks at 4 degrees C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The immobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the immo...

Directional sensing by eukaryotic cells does not require polarization of chemoattractant receptors. The translocation of the PH domain-containing protein CRAC in D. discoideum to binding sites on the inner face of the plasma membrane reflects activation of the G protein-linked signaling system. Increments in chemoattractant elicit a uniform response around the cell periphery. Yet when cells are...

Immobilization is the method of entrapping/attaching the microbial or plant cells in a suitable matrix. Different methods such as encapsulation, gel entrapment, covalent bonding, cross linking and adsorption is carried out to prepare immobilized cells. When compared to enzyme immobilization, cell immobilization is regarded as a convenient method because of its low cost and improved stability. T...

Variants of malignant RAW117 lymphosarcoma cell lines were selected by sequential nonadherence to polystyreneImmobilized lectlns. Parental murine lymphosarcoma line RAW117-P grew In suspension and was rapidly bound by Immobilized concanavalin A (Con A), Ricinus communis agglutinin I (RCAI), peanut agglutinin (PNA), or wheat germ agglutinin (WGA). After attachment of most cells to lectin-substit...