Measles is a highly contagious respiratory virus infection, with typical clinical symptoms including maculopapular rash, fever, cough, coryza, and conjunctivitis. Despite implementation of widespread vaccination programs throughout the world, the rates of global morbidity and mortality are still considerable. This study was performed to design a reliable indirect enzyme-linked immunosorbent ass...

Serological tests for Toxoplasma gondii are inadequate because antibody production either fails or is significantly delayed. This study in eastern Iraq investigated the IgG-avidity ELISA test for detecting recent T. gondii infections among pregnant women and compared immunological methods and PCR as molecular assays in the diagnosis of T. gondii. Serums samples were taken from 130 pregnant wome...

INTRODUCTION Dengue and leptospirosis are two febrile illnesses of great clinical and epidemiological importance in Brazil. Their significant degree of symptomatic similarity makes clinical diagnosis difficult. OBJECTIVE To diagnose leptospirosis differentially in patients with clinically suspected dengue. MATERIALS AND METHODS In this study, 86 patients with clinically suspected dengue und...

Detection of Japanese encephalitis virus (JEV)-specific IgM by IgM-capture enzymed-linked immunosorbent assay (IgM-capture ELISA) has been accepted as the standard for serological diagnosis. In the present study, we analyzed the time course of the positive rate of JEV-specific IgM in serum and cerebrospinal fluid (CSF) specimens from confirmed JE patients. Serum and CSF samples were obtained fr...

BACKGROUND AND OBJECTIVES Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Because of the difficulty in the diagnosis of brucellosis, particularly in endemic areas, the use of new and feasible diagnostic tests seem to be of great importance for resolving the diagnostic obstacles. We evaluated the usefulness of a new se...

Background: A test strip IgM Dot-ELISA assay for the detection of leptospirespecific IgM antibodies in human sera was developed. Antigen dotted on a nitrocellulose paper strip was the sonicated antigen prepared from Leptospira serovar prevalent in this area, i.e., Icterohaemmorhagiae. Objective: The aim of this study was to evaluate an inhouse prepared IgM DotELISA test strip for the diagnosis ...

Commercial chikungunya virus (CHIKV)-specific IgM detection kits were evaluated at the Centers for Disease Control and Prevention (CDC), the Public Health Agency of Canada National Microbiology Laboratory, and the Caribbean Public Health Agency (CARPHA). The Euroimmun Anti-CHIKV IgM ELISA kit had ≥ 95% concordance with all three reference laboratory results. The limit of detection for low CHIK ...

background: toxoplasmosis is a zoonotic disease with widespread distribution throughout the world. it is caused by the pro­tozoan parasite toxoplasma gondii . as laboratory diagnosis of toxoplasmosis is not straightforward, this study was aimed to compare the sensitivity and specificity of conventional and unconventional methods of diagnosis based on the meas­urement of igm and igg for determin...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is sweeping the world since end of 2019. The titer change antibodies against SARS-CoV-2 needs to be further clarified, clinical and preventive value still investigated. An enzyme-linked immunosorbent assay (ELISA) was established by coating with recombinant spike protein used detect serum immunoglobulin M (IgM) G (IgG) in disease 2019...

Background: Dengue is an acute viral infection has emerged as a notable public health problem. Rapid immunochromatographic test and IgM/IgG ELISA been the mainstay of diagnosis. Combination NS1 antigen detection along with antibody increases diagnostic rates. Thrombocytopenia begins during febrile phase. Therefore, we tried to evaluate association platelet counts against (NS1 IgM/IgG) IgM in de...