A direct enzyme-linked immunosorbent assay (ELISA) is described that uses horseradish peroxidase-labeled antigen for detection of immunoglobulin M (IgM) and IgA antibodies to toxoplasma. In this assay, polystyrene microtiter plates were sensitized with anti-human IgM or IgA antibody to separate IgM or IgA from other classes of antibody. The presence of IgM or IgA antibodies to toxoplasma (Tox-I...

Monomeric (75) IgM was measured in 25 patients with primarybiliary cirrhosis and 25 ageand se~matched controls by simpledouble immunodiffusion in a polyacrylamide gel. 75 IqM was detectedin a quarter of the patients and~_none of the controls.The presence of 75 IgM was related to the elevation of totalIgM as measured by the radial immunodiffusion techniques.Patients with ...

some arrangement of inter-␮ bonds directs incorporation of J chain. IgM-S337 hexamer also dissociated to ␮2␬2 and ␮4␬4, but also yielded several species migrating much more slowly in SDS-PAGE than wild-type ␮12␬12. To account for these forms, we propose that each ␮-chain can interact with three other ␮-chains and that some hexameric molecules contain two catenated ␮6␬6 circles. J chain is a 15-...

The glycoprotein IgM is the major antibody produced in the primary immune response to antigens, circulating in the serum both as a pentamer and a hexamer. Pentameric IgM has a single J chain, which is absent in the hexamer. The mu (heavy) chain of IgM has five N-linked glycosylation sites. Asn-171, Asn-332, and Asn-395 are occupied by complex glycans, whereas Asn-402 and Asn-563 are occupied by...

background: toxoplasmosis is well known as an important infection in pregnant women. although many serologic methods are available, diagnosis of early toxoplasmosis may be extremely difficult. objective: to detect the toxoplasma igg antibodies developed at the early stage of infection in pregnant women. methods: 225 pregnant women, who were in the 2nd to 4th month of their pregnancy, enrolled i...

as igm and iga-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of igm and iga enriched immunoglobulins. fraction iii which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using sephadex deae a-50 and 0.1 m tris-d.35m naci buff...

INTRODUCTION In this investigation we studied clinical and laboratory features of polyneuropathies in patients with serum IgM binding to the trisulfated disaccharide IdoA2S-GlcNS-6S (TS-HDS). METHODS We retrospectively compared 58 patients with selective IgM binding to TS-HDS to 41 consecutive patients with polyneuropathies without TS-HDS binding. RESULTS Patients with IgM vs. TS-HDS common...