objective: garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. infectious bronchitis virus (ibv) is a coronavirus. the available vaccines against ibv cannot cover new variants. this study evaluated the inhibitory effects of garlic extract on ibv.  materials and methods: the constituents of garlic extract were detected by gas chromatography. this study was d...

The aim of this study was to provide information on the molecular characteristic and the phylogenic relationship of infectious bronchitis viruses (IBV) strains in Bushehr province in comparison to other strains reported in the Middle East. Samples were collected from broiler flocks in Bushehr province during 2014 - 2015. These flocks had respiratory problems such as gasping, sneezing and bronch...

The susceptibility of the primary chick embryo chorioallontoic membrane cells to infectious bronchitis virus was evaluated after twenty consecutive passages in chick embryo chorioallontoic membrane cells. Virus replication was monitored by cytopathic observation, indirect immunoperoxidase, and reverse transcription polymerase chain reaction (RT-PCR). At 72 hours post-infection (p.i.) in third p...

Avian infectious bronchitis has caused huge economic losses in the poultry industry. Previous studies have reported that infectious bronchitis virus (IBV) infection can produce cytopathic effects (CPE) and apoptosis in some mammalian cells and primary cells. However, there is little research on IBV-induced immune cell apoptosis. In this study, chicken macrophage HD11 cells were established as a...

the aim of the present study was using rt-pcr for the diagnosis of avian infectious bronchitis virus and massachusetts serotype in tissue samples. optimization of a molecular diagnostic method for the detection of avian bronchitis virus and identification of massachusetts serotype was investigated. in order to detect infectious bronchitis virus (ibv) in tissue samples, an rt-pcr was optimized. ...

Infectious bronchitis virus (IBV) is prevalent in all countries with an intensive poultry industry, with the incidence of infection approaching 100% in most locations. Vaccination is only partially successful due to the continual emergence of antigenic variants. At many sites, multiple antigenic types are simultaneously present, requiring the application of multiple vaccines. Although many coun...

Abstract Avian infectious bronchitis (IB) is a highly contagious disease caused by virus (IBV), coronavirus of domestic fowl. IB major concern in the poultry industry, causing worldwide economic losses through decreased egg production and quality increasing chicken's susceptibility for secondary bacterial infections, particularly Escherichia coli . In this study, anti‐IBV activity cathelicidins...

Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these IBV isolates were amplified by reverse transcriptase-polymerase chain reaction (RTPCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. These Korean IBV isolates were cl...

The coronavirus (CoV) E protein plays an important role in virus assembly. The E protein is made in excess during infection and has been shown to have ion channel activity in planar lipid bilayers. However, a role in infection for the unincorporated E or its ion channel activity has not been described. To further investigate the function of the infectious bronchitis virus (IBV) E protein, we de...

This study was designed to examine the impact of competitive non-protective antigens in a bivalent killed vaccine of Newcastle disease virus and infectious bronchitis (IB) virus on seroconversions against protective fusion protein of Newcastle disease (ND) virus (NDV), in free-range layers primed by live ND-clone 30 and IB-H120 vaccines. The experimental design included two free-range layer far...