background: since alkaloids are nitrogenous compounds, the availability of nitrogen (n) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. objective: this study intended to investigate the nitrogen (n) fertilization and water deficit stress (wds) effects on seed tropane alkaloids elicitation including hyoscyamine (hyo) and scopolamine (sco), and a...

background: regarding the prevalence of epilepsy in human society and with respect to inefficiency of the usual treatments, finding new strategies and methods for the medical treatment of epileptic patients are essential. henbane seed has been used in iranian traditional medicine as an anti-convulsion herb. with this regard the present study was carried out to consider the anti-convulsive effec...

BACKGROUND AND AIMS Ibicella lutea and Proboscidea parviflora are two American semi-desert species of glandular sticky plants that are suspected of carnivory as they can catch small insects. The same characteristics might also hold for two semi-desert plants with glandular sticky leaves from Israel, namely Cleome droserifolia and Hyoscyamus desertorum. The presence of proteases on foliar hairs,...

the purpose of the present study was to determine the tropane alkaloid content of four hyoscyamus species from iran, i.e. h. pusillus l., h. niger l., h. reticulatus l. and h. kurdicus bornm. determination of alkaloids was performed by the hplc method. samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (v/v/v). hplc separation was performed on eurospher c18 reversed p...

The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...

Background & Aims of the Study: Malaria is an infectious disease by fever, chills and anemia; and splenomegaly genus Plasmodium parasite is the agent of it. One of the easiest and least expensive methods for preventionof this disease is removing the vector that usually has been done by insecticides and chemical pesticides, but now days due to the harmful effects of toxic chemic...

Two stereospecific NADPH-dependent reductases, TR-I and TR-11, constitute a branching point in the biosynthesis of tropane alkaloids. TR-I catalyzes the stereospecific reduction of tropinone to tropine (Koelen and Gross, 1982), whereas TR-I1 reduces tropinone to pseudotropine (Drager et al., 1988). We previously characterized TRs that had been purified from cultured roots of Hyoscyamus niger (H...

Hyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron-deficient condition secreted riboflavin from the root tips into the culture medium and the productivity depended on the number and size of root tips among the clones. A decline of pH was observed before riboflavin production and root...